NEW Agilent 4 um AdvanceBio Oligonucleotide Columns

AdvanceBio Oligonucleotide columns offer high-quality, high-resolution ion-pair reversed-phase (IP-RP) column chemistry platform that support analytical characterization through purification with seamless method scale-up. To successfully separate trityl-off, deprotected oligonucleotides, HPLC columns need to have high resolving power and be robust enough to withstand high pH and temperature, which provide better separation and denaturing conditions for optimal resolution and oligonucleotide separation.

AdvanceBio Oligonucleotide columns feature high efficiency, 2.7 and 4 μm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. In addition, we test every batch of AdvanceBio Oligonucleotide media with a resolution standard to ensure consistent performance. AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization and bioanalysis of proteins, antibodies, conjugates, new biological entities and biopharmaceuticals.

 

Key Features of the 4 µm SPP particle:

>  Same chemistry as 2.7µm particle, but with lower back pressure

>  Higher efficiency and increased resolution compared to 5 µm FPP alternative with system optimization

>  Scalable particle eliminates need for chemistry re-validation when scaling up to purification

>  Increased resolution of target product from closely related impurities (n+/- 1) Improved yield and purity of target product

 

Download the AdvanceBio Oligonucleotide Preparative Purification Application Note for more information.