Quality Bio RPMI-1640 w/o Phenol Red & L-Glutamine

Quality Bio Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures.RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). Common Applications of RPMI-1640 include: fusion protocols and growth of hybrid cells, culture of normal human leukocytes in monolayer and suspension, and support of HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinoma growth (to name a few).RecommendationFor those downstream protein purification studies, or other processes where the presence of phenol red is undesirable. Phenol red has been shown to interfere with the growth of some cells at clonal densities; use this medium when working with stem cells or when growing cell at low densities Phenol red may interfere with some chromatography processes involved with downstream purification. Storage Conditions: 2Deg.C to 8Deg.C Shipping Condition: ambient temperatureSterile FilteredpH: 7.0 ± 0.2Osmolality: 300 ± 5% mOsm/Kg H2OProduct and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental healthHarmonization Code: 3821.00.0000ECCN: EAR99Max Shelf Life: 24 months - QB (Additional S&H or Hazmat Fees Will Apply)