IBI Scientific Gel/PCR DNA Fragment Extraction Kit
IBI Sci PCR DNA FRAG. EXTRACTION KIT 100 PREP KIT (additional Shipping and HAZMAT may apply) - IBI
- Sample size: up to 300mg agarose gel/up to 100µl PCR products
- Binding capacity: 10µg DNA
- Expectant Yield: 80-90% for gel extraction/90-95% for PCR Clean-Up
- Operation Time: 20min or less
The IBI PCR Clean-up and DNA Fragment Gel Extraction Kit (IB47020) is designed to recover or concentrate DNA fragments (50bp – 10Kb) from agarose gel, PCR, or other enzymatic reactions. This method uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass fiber matrix of the spin column.
The contaminants are washed with a wash buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt elution buffer or pure water. Salts, enzymes or unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are 90 – 95% for PCR clean-up.
The entire procedure can be completed in 20 minutes, and the eluted DNA is ready for use in restriction digestion, ligation, PCR, fluorescent or radioactive sequencing, and DNA labeling.
This kit will perform both PCR clean-up and gel extraction procedures, eliminating the need for two kits. - IBI
|Manufacturer Part Number||IB47020|
|Unit Of Measure||UN|
|Manufacturer Name||IBI Scientific|
|Quantity Per UOM||1|