What is your starting material?
RNA
What do you value most in a reverse transcriptase?
Accuracy
What is your preferred format?
Stand Alone Enzyme
Recommendation:
AccuScript High-Fidelity Reverse Transcriptase
Product Details
-
Highest reverse transcription accuracy with 3-6x fewer errors
-
Full length cDNA up to 20 kb
-
Up to 3x faster RT-PCR reaction times
Ordering Info
Size |
Catalog |
50 rxn |
600089 |
200 rxn |
600090 |
RT-PCR Kit
Recommendation:
AccuScript PfuUltra II RT-PCR Kit
Product Details
-
Complete system for RT-PCR applications that require high-fidelity, specificity & throughput
-
Amplifies cDNA targets up 9.6 kb in length
-
Synthesize cDNA from 10-1000 ng of total RNA or 0.1-10 ng of poly(A)+ RNA
-
Up to 3x faster RT-PCR reaction times
Ordering Info
Size |
Catalog |
50 rxn |
600184 |
Robustness/Yield
What is your preferred format?
Stand Alone Enzyme
Recommendation:
AffinityScript Multiple Temperature Reverse Transcriptase
Product Details
-
Robust cDNA synthesis across a broad temperature range,
37-55°C
-
High affinity for primer-template complexes promotes optimal yield
-
Synthesize long cDNA targets, up to 20 kb
-
Detects low input RNA
Ordering Info
Size |
Catalog |
10 rxn |
600105 |
50 rxn |
600107 |
200 rxn |
600109 |
RT-PCR Kit
Recommendation:
AffinityScript One-Step RT-PCR Kit
Product Details
-
Reduces cycling times & contamination risk with a one-tube, master mix format
-
High affinity for primer-template complexes promotes optimal yield
-
Robust performance for difficult and GC-rich targets
-
System optimized for target lengths up to 9.5 kb
Ordering Info
Size |
Catalog |
100 rxn |
600188 |
DNA
Select the enzyme category that best fits your needs:
High-Fidelity (Proofreading)
Select the best description for your target:
GC-rich or long templates
Recommendation:
Herculase II Fusion DNA Polymerases
Product Details
-
Superior yield for routine PCR applications & difficult/GC-rich targets
-
Fast cycling time with 15 sec/kb extension rate
-
Accurately amplifies up to 23 kb genomic DNA targets
-
Preserves Pfu fidelity, 1 error/770,000 bp
-
High sensitivity for amplification of low abundance targets
Ordering Info
Size |
Catalog |
40 rxn |
600675 |
200 rxn |
600677 |
400 rxn |
600679 |
Template contains Uracil and/or dUTP in the PCR Reaction
Recommendation:
PfuTurbo Cx HotStart DNA Polymerase
Product Details
-
World's only high-fidelity polymerase that reads through and incorporates dUTP
-
Great for bi-sulfite sequencing, UNG decontamination protocols & DNA methylation studies
-
Accurately amplifies up to 10 kb genomic DNA templates, including GC-rich targets
-
DMSO provided separately to assist in optimizing PCR conditions
Ordering Info
Size |
Catalog |
100 U |
600410 |
500 U |
600412 |
1000 U |
600414 |
PCR product intended for cloning
Would you prefer the most accurately amplified PCR products or products
ready for TA/UA cloning?
Most Accurate (Highest Fidelity)
What is your preferred format?
Hot Start Master Mix
Recommendation:
PfuUltra II HotStart Master Mix
Product Details
-
Highest fidelity for the most reliable, error-free PCR
-
Fast cycling time with 15 sec/kb extension rate
-
Accurately amplifies up to 19 kb genomic DNA targets
-
Ideal for applications such as cloning, protein crystallization studies & sequencing
Ordering Info
Size |
Catalog |
100 rxn |
600850 |
400 rxn |
600852 |
Hot Start Enzyme
Recommendation:
PfuUltra II Fusion HotStart DNA Polymerase
Product Details
-
Highest fidelity for the most reliable, error-free PCR
-
Fast cycling time with 15 sec/kb extension rate
-
Accurately amplifies up to 19 kb genomic DNA targets
-
Ideal for applications such as cloning, protein crystallization studies & sequencing
Ordering Info
Size |
Catalog |
40 rxn |
600670 |
200 rxn |
600672 |
400 rxn |
600674 |
Most Convenient (TA/UA Cloning
Ready)
What is your preferred format?
Hot Start Master Mix
Recommendation:
Easy-A High-Fidelity PCR Master Mix
Product Details
-
Combines accuracy of Pfu with TA/UA cloning efficiency of Taq
-
Preferentially adds a single 3’-A overhang to PCR products
-
PCR products have greater than 95% cloning efficiency
Ordering Info
Size |
Catalog |
100 rxn |
600640 |
400 rxn |
600642 |
Hot Start Enzyme
Recommendation:
Easy-A High-Fidelity PCR Cloning Enzyme
Product Details
-
Combines accuracy of Pfu with TA/UA cloning efficiency of Taq
-
Preferentially adds a single 3’-A overhang to PCR products
-
PCR products have greater than 95% cloning efficiency
Ordering Info
Size |
Catalog |
100 U |
600400 |
500 U |
600402 |
1000 U |
600404 |
Minimal template DNA (low copy number, archaic, precious samples)
Recommendation:
PicoMaxx High-Fidelity PCR System
Product Details
-
Highest sensitivity to detect low copy number targets
-
Robustly withstands the effects of PCR inhibitors
-
Robust amplification of genomic DNA targets up to 10 kb
-
High-specificity hot start increases enzyme sensitivity
Ordering Info
Size |
Catalog |
100 U |
600420 |
500 U |
600422 |
1000 U |
600424 |
Master Mix (100 rxn) |
600650 |
Herculase II Fusion DNA Polymerase
Product Details
-
Superior yield for routine PCR applications & difficult/GC-rich targets
-
Fast cycling time with 15 sec/kb extension rate
-
Accurately amplifies up to 23 kb genomic DNA targets
-
Preserves Pfu fidelity,
1 error/770,000 bp
-
High sensitivity for amplification of low abundance targets
Ordering Info
Size |
Catalog |
40 rxn |
600675 |
200 rxn |
600677 |
400 rxn |
600679 |
Other
What do you value the most in DNA Polymerase?
Accuracy
What is your preferred format?
Hot Start Master Mix
Recommendation:
PfuUltra II HotStart Master Mix
Product Details
-
Highest fidelity for the most reliable, error-free PCR
-
Fast cycling time with 15 sec/kb extension rate
-
Accurately amplifies up to 19 kb genomic DNA targets
-
Ideal for applications such as cloning, protein crystallization studies & sequencing
Ordering Info
Size |
Catalog |
100 rxn |
600850 |
400 rxn |
600852 |
Hot Start Enzyme
Recommendation:
PfuUltra II Fusion HotStart DNA Polymerase
Product Details
-
Highest fidelity for the most reliable, error-free PCR
-
Fast cycling time with 15 sec/kb extension rate
-
Accurately amplifies up to 19 kb genomic DNA targets
-
Ideal for applications such as cloning, protein crystallization studies & sequencing
Ordering Info
Size |
Catalog |
40 rxn |
600670 |
200 rxn |
600672 |
400 rxn |
600674 |
Robustness/Yield
Recommendation:
Herculase II Fusion DNA Polymerase
Product Details
-
Superior yield for routine PCR applications & difficult/GC-rich targets
-
Fast cycling time with 15 sec/kb extension rate
-
Accurately amplifies up to 23 kb genomic DNA targets
-
Preserves Pfu fidelity, 1 error/770,000 bp
-
High sensitivity for amplification of low abundance targets
Ordering Info
Size |
Catalog |
40 rxn |
600675 |
200 rxn |
600677 |
400 rxn |
600679 |
Fidelity
Recommendation:
PfuUltra II Fusion Hot Start DNA Polymerase
Product Details
-
Highest fidelity with great PCR success and reliability
-
70 to 80 % quicker time to result
-
High specificity hotstart formulation
-
Amplify up to 19 kb genomic DNA
-
Ideal for PCR cloning, RT-PCR, and site-directed mutagenesis
Ordering Info
Size |
Catalog |
40 rxn |
600670 |
200 rxn |
600672 |
400 rxn |
600674 |
Non-Proofreading
What is your preferred format?
Hot Start Master Mix
Recommendation:
Paq5000 2X PCR Master Mix
Product Details
-
Robust, cost effective alternative to Taq
-
Faster cycling times with 30 sec/kb extension rate
-
Amplify up to 6 kb genomic DNA targets
Ordering Info
Size |
Catalog |
100 rxn |
600870 |
400 rxn |
600872 |
Hot Start Enzyme
Recommendation:
SureStart Taq DNA Polymerase
Product Details
-
Hot start for greater PCR specificity and yields
-
Virtually eliminates background artifacts and smearing in long PCR
-
Reliable room-temperature setup
-
Ultrapure cloned Taq DNA polymerase
Ordering Info
Size |
Catalog |
100 U |
600280 |
500 U |
600282 |
1000 U |
600284 |
Stand Alone Enzyme
Recommendation:
Taq2000 DNA Polymerase
Product Details
-
Ultrapure cloned Taq DNA polymerase
-
Virtually eliminates background artifacts and smearing in long PCR
-
Most thermostable Taq DNA polymerase
Ordering Info
Size |
Catalog |
100 U |
600195 |
500 U |
600196 |
1000 U |
600197 |
5000 U |
600198 |
Welcome to the Polymerase Decision Tree!
This decision tree is your personal guide through Agilent's PCR enzyme portfolio. In just a few clicks, find out which polymerase best fits your application.
GET STARTED!
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