Are you transforming for protein expression, cloning, phage display or random mutagenesis?
Protein Expression
Will you be using a T7 promoter?
Yes
Do you want your protein product to be phosphorylated?
Yes
Recommended Product:
TKB1 Competent Cells
Product Details
Derived from BL21 strain, use with pET, pCAL or other T7 RNA promoter-based vectors.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200134 |
No
Will you be expressing an Eukaryotic protein and anticipate a codon bias?
Yes
Do you anticipate that your genes will have any of the following?
High GC
Will the protein require radio labeling?
Yes
Recommended Product:
BL21-CodonPlus (DE3)-RP-X Competent Cells
Product Details
Encodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression plus methionine label, Use with pET or pCAL vectors, Used for labeling cells 1 x 107 cfu/µg of supercoiled DNA.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230275 |
No
Do you anticipate solubility problem?
Yes
Recommended Product:
ArcticExpress (DE3) RP Competent Cells
Product Details
Enhanced protein folding and solubility of expressed proteins. Dramatically improves expression for GC-rich genomes when codon bias is a problem. Encodes T7 RNA polymerase under control of the lacUV5 promoter.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230194 |
No
Recommended Product:
BL21-CodonPlus (DE3)-RP Competent Cells
Product Details
Encodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression, Use with pET or pCAL vectors, 1 x 107 cfu/µg of supercoiled DNA.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230255 |
High AT
Will the protein require radio labeling?
Yes
Recommended Product:
BL21-CodonPlus (DE3)-RIL-X Competent Cells
Product Details
Encodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression plus methionine label, Use with pET or pCAL vectors, Used for labeling cells 1 x 107 cfu/µg of supercoiled DNA.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230265 |
No
Do you anticipate solubility problem?
Yes
Recommended Product:
ArcticExpress (DE3) RIL Competent Cells
Product Details
Enhanced protein folding and solubility of expressed proteins. Dramatically improves expression for AT-rich genomes when codon bias is a problem. Encodes T7 RNA polymerase under control of the lacUV5 promoter.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230193 |
No
Recommended Product:
BL21-CodonPlus (DE3)-RIL Competent Cells
Product Details
Encodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression, Use with pET or pCAL vectors, 1 x 107 cfu/µg of supercoiled DNA.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230245 |
Unsure
Recommended Product:
BL21-CodonPlus (DE3)-RIPL Competent Cells
Product Details
These cells contain extra copies of rare E. coli argU, ileY, leuW and proL tRNA genes which corrects for codon bias and dramatically improves expression of sequences from other organisms.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230280 |
No
Have you experienced difficulties producing the expected yield?
Yes
Do you expect the problem to be with toxicity or solubility?
Toxicity
Are you inducing with IPTG or λ induction?
IPTG
BL21-Gold (DE3)pLysS Competent Cells
Product Details
For both toxic and non-toxic proteins, pLysS codes for T7 lysozome, a T7 RNA polymerase inhibitor. Use with Variflex or Affinity pCAL vectors. Efficiency is ≥1 x 108 cfu/ µg of supercoiled DNA.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230134 |
BL21(DE3) pLysS Competent Cells
Product Details
For both toxic and non-toxic proteins, pLysS codes for T7 lysozyme, a T7 RNA polymerase inhibitor. Use with pET or pCAL vectors.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200132 |
λ induction
BL21-Gold Competent Cells
Product Details
For extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based system. Efficiency is ≥1 x 108 cfu/µg of supercoiled DNA.
Lambda CE6 Induction Kit
Delivers T7 RNA polymerase into BL21 competent cells to induce expression of toxic proteins from T7 promoter-driven vectors. Allows for tight control of protein expression.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230130 |
| 1 Kit (1.0 mL lambda CE6) | 235200 |
BL21 Competent Cells
Product Details
For extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based systems
Lambda CE6 Induction Kit
Delivers T7 RNA polymerase into BL21 competent cells to induce expression of toxic proteins from T7 promoter-driven vectors. Allows for tight control of protein expression.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200133 |
| 1 Kit (1.0 mL lambda CE6) | 235200 |
Solubility
Recommended Product:
ArcticExpress (DE3) Competent Cells
Product Details
Enhanced protein folding and solubility of expressed proteins. Encodes T7 RNA polymerase under control of the lacUV5 promoter.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230192 |
No
BL21-Gold (DE3) Competent Cells
Product Details
For non-toxic proteins, encodes T7 RNA polymerase under the control of the lacUV5 promoter. Use with Variflex or Affinity pCAL vectors. Efficiency is ≥1 x 108 cfu/µg of supercoiled DNA.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230132 |
BL21(DE3) Competent Cells
Product Details
For non-toxic proteins, encodes T7 RNA polymerase under the control of the lacUV5 promoter. Use with Variflex or Affinity pCAL vectors.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200131 |
No
Do you want your protein product to be phosphorylated?
Yes
TKX1 Competent Cells
Product Details
Derived from XL1-Blue strain, use with non-T7 promoter based vectors.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200124 |
No
Have you experienced difficulties producing the expected yield?
Yes
Do you get a very low amount of protein,
or enough protein but it is insoluble (in pellet)?
or enough protein but it is insoluble (in pellet)?
Solubility and possibly Codon Bias
Will you be expecting Codon Bias?
Yes
Will you be expecting High AT or High GC?
High AT
Recommended Product:
ArcticExpress RIL Competent Cells
Product Details
Enhanced protein folding and solubility of expressed proteins. Dramatically improves expression for AT-rich genomes when codon bias is a problem.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230195 |
High GC
Recommended Product:
ArcticExpress RP Competent Cells
Product Details
Enhanced protein folding and solubility of expressed proteins. Dramatically improves expression for GC-rich genomes when codon bias is a problem.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230196 |
No
Recommended Product:
ArcticExpress Competent Cells
Product Details
Enhanced protein folding and solubility of expressed proteins.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230191 |
Codon Bias only
Do you expect high GC or high AT?
High GC
Recommended Product:
BL21-CodonPlus-RP Competent Cells
Product Details
For extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase,1 x 107 cfu/µg of supercoiled DNA.
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230250 |
High AT
Recommended Product:
BL21-CodonPlus -RIL Competent Cells
Product Details
For extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase,1 x 107 cfu/µg of supercoiled DNA.
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230240 |
No
Recommended Product:
XL1-Red Competent Cells
Product Details
For random mutagenesis, 1 x 106 transformants/µg, includes XL1-Blue competent cellsOrdering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200129 |
No
Do you want higher transformation efficiency?
Yes
Recommended Product:
BL21-Gold Competent Cells
Product Details
For extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based system. Efficiency is ≥1 x 108 cfu/µg of supercoiled DNA.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 230130 |
No
Recommended Product:
BL21 Competent Cells
Product Details
For extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based systems.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200133 |
Cloning
Will you be transforming large, unstable or toxic plasmids?
Yes
Are they large, unstable or toxic?
Large
Do you have an electroporater?
Yes
EletroTen-Blue Electroporation-Competent Cells
Product Details
Highest electroporation efficiency (Hee) phenotype, use for extrememly demanding cloning,≥ 3 x 1010 transformants/µg.
Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.1 mL | 200159 |
XL1-Blue Electroporation-Competent Cells
Product Details
For cloning unmethylated DNA,≥1 x 1010 transformants/µg.
Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.1 mL | 200228 |
No
Do you require tetracycline Sensitivity?
Yes
Recommended Product:
XL10-Gold Kanr Ultracompetent Cells
Product Details
Features the kanamycin-resistance gene on the F' episome, for extremely demanding cloning in chloramphenicol-resistant vectors. Efficiency: > 5 x 109 transformants/µg pUC18 DNA..Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 200317 |
No
XL10-Gold Ultracompetent Cells
Product Details
For extremely demanding cloning, large plasmids and plasmid libraries. Efficiency: ≥5 x 109 transformants/µg pUC18 DNA Contains: XL10-Gold ultracompetent cells, pUC18 control plasmid, XL10-Gold b-mercaptoethanol mix.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.1 mL | 200314 |
XL10-Gold Ultracompetent Cell
Product Details
For extremely demanding cloning, large plasmids and plasmid libraries. Efficiency: ≥ 5 x 109 transformants/µg pUC18 DNA Contains: XL10-Gold ultracompetent cells, pUC18 control plasmid, XL10-Gold b-mercaptoethanol mix.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 200315 |
Unstable
Is your plasmid instability due to toxicity or recombination?
Toxicity
Do you have an electroporater?
Yes
Recommended Product:
ABLE Electroporation-Competent Cell Kit
Product Details
Includes both ABLE C and K cells, Contains: 5 x 0.1 mL ABLE C cells, 5 x 0.1 mL ABLE K cells.Ordering Info
| Size | Catalog |
|---|---|
| 1 Kit | 200160 |
No
Recommended Product:
ABLE Competent Cell Kit
Product Details
Includes both ABLE C and K cells, Contains: 5 x 0.2 mL ABLE C and 5 x 0.2 mL ABLE K cells.Ordering Info
| Size | Catalog |
|---|---|
| 1 Kit | 200170 |
Recombination
Do you have an electroporater?
Yes
Recommended Product:
SURE Electroporation-Competent Cells
Product Details
For cloning DNA with secondary structures, ≥1 x 1010 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.1 mL | 200227 |
No
Will you have limited quantity of DNA for transformation?
Yes
Recommended Product:
SURE 2 Supercompetent Cells
Product Details
High-efficiency derivative for cloning DNA with secondary structures, > 1 x 109 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 200152 |
No
Recommended Product:
SURE Competent Cells
Product Details
For routine cloning of DNA with secondary structures, ≥5 x 108 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200238 |
Toxic
Do you need electroporatable cells or chemically competent cells?
Electroporatable cells
What degree of toxicity do you expect: Mildly toxic or more toxic?
Mildly Toxic
(4-fold reduction of copy number)
(4-fold reduction of copy number)
Recommended Product:
ABLE C Electroporation-Competent Cells
Product Details
Reduces the number of common cloning vectors 4-fold.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.1 mL | 200161 |
More Toxic
(10-fold reduction of copy number)
(10-fold reduction of copy number)
Recommended Product:
ABLE K Electroporation-Competent Cells
Product Details
Reduces the number of common cloning vectors 4-fold.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.1 mL | 200162 |
Chemically Competent
What degree of toxicity do you expect: Mildly toxic or more toxic?
Mildly Toxic
(4-fold reduction of copy number)
(4-fold reduction of copy number)
Recommended Product:
ABLE C Competent Cells
Product Details
Reduces the number of common cloning vectors 4-fold.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200171 |
More Toxic
(10-fold reduction of copy number)
(10-fold reduction of copy number)
Recommended Product:
ABLE K Competent Cells
Product Details
Reduces copy number of common cloning vectors by 10-fold, allowing toxic clones to replicate.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200172 |
No
Do you need make unmethylated DNA?
Yes
Recommended Product:
SCS110 Competent Cells
Product Details
≥ 5 x 106 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200247 |
JM110 Competent Cells
Product Details
≥ 5 x 106 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200239 |
No
Will you be cloning Genomic DNA or cDNA?
Yes
Do you have an electroporater?
Yes
Recommended Product:
XL1-Blue MRF’ Electroporation-Competent Cells
Product Details
Restriction minus for cloning methylated DNA, ≥ 1 x 1010 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.1 mL | 200158 |
No
Do you have limited quantity of plasmid DNA?
Yes
Do you need a host with no antibiotic resistance?
Yes
XL1-Blue MR Supercompetent Cells
Product Details
For use with cloning without the F' episome, ≥ 1 x 109 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200229 |
SCSI Supercompetent Cells, 5 x 0.2 mL
Product Details
1 x 109 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200231 |
No
Recommended Product:
XL2-Blue MRF’ Ultracompetent Cells
Product Details
Restriction minus for cloning methylated DNA, highest cloning efficiency, > 5 x 109 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 200151 |
No
Do you require Chloramphenicol for selection?
Yes
Recommended Product:
XL1-Blue MRF’ Kan Supercompetent Cells
Product Details
Large aliquot size for scale-up library transformations, ≥1 x 109 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200248 |
No
Recommended Product:
XL1-Blue MRF’ Supercompetent Cells
Product Details
Restriction minus for cloning methylated DNA, ≥ 1 x 109 transformants/µg.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200230 |
No
Will your quantity of templates will be limited?
Yes
XL2-Blue Ultracompetent Cells
Product Details
Highest cloning efficiency,> 5 x 109 transformants/µg.
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 0.1 mL | 200150 |
XL1-Blue Supercompetent Cells
Product Details
For high-efficiency cloning,≥1 x 109 transformants/µg.
Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200236 |
No
XL1-Blue Subcloning Grade Competent Cell
Product Details
For cloning where DNA is not limited, ≥ 1 x 106 transformations/µg.Ordering Info
| Size | Catalog |
|---|---|
| 8 x 0.5 mL | 200130 |
Phage Display
Recommended Product:
TG1 Electroporation-Competent Cells
Product Details
For use in phage display library preparation, ≥1 x 1010 transformations/µg.Ordering Info
| Size | Catalog |
|---|---|
| 5 x 0.1 mL | 200123 |
Random Mutagenesis
Recommended Product:
XL1-Red Competent Cells
Product Details
For random mutagenesis, 1 x 106 transformants/µg, includes XL1-Blue competent cellsOrdering Info
| Size | Catalog |
|---|---|
| 5 x 0.2 mL | 200129 |
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