What is your starting material?
DNA
What chemistry will you be using?
Probes
What is your preferred cycling format?
Fast
Recommended Product:
Brilliant III Ultra-Fast QPCR Master Mix
Product Details
- Extremely fast while maintaining sensitivity
- Greater resistance to common QPCR inhibitors
- Optimized fast cycling formulation
- Compatible with any sequence-specific probe detection chemistry
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600880 |
Brilliant III Ultra-Fast QPCR Master Mix
Product Details
- Extremely fast while maintaining sensitivity
- Greater resistance to common QPCR inhibitors
- Optimized fast cycling formulation
- Compatible with any sequence-specific probe detection chemistry
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 400 Rxn | 600881 |
Standard
Are you planning to multiplex with 3 or more targets?
Yes
Recommended Product:
Brilliant Multiplex QPCR Master Mix
Product Details
- Maximizes analysis of limited/rare samples
- Allows detection of multiple targets + an internal control
- Cost Effective
Ordering Info
| Size | Catalog |
|---|---|
| 200 Rxn | 600553 |
No
Do you want ROX included in the Master Mix?
Yes
Do you want a Master Mix with High ROX (eg laser based instrument) or Low ROX (eg MX 3005P)?
Low ROX
Recommended Product:
Brilliant II qPCR Low ROX Master Mix
Product Details
- Formulations available with low and high concentrations of ROX reference dye
- Earlier Ct detection across a wide dynamic range
- Increased sensitivity of detection to low copy numbers
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600806 |
High ROX
Recommended Product:
Brilliant II qPCR High ROX Master Mix
Product Details
- Improved performance with earlier Ct detection
- Increased sensitivity of detection to low copy numbers
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600805 |
No
Recommended Product:
Brilliant II QPCR Master Mix
Product Details
- Greater flexibility (numerous different templates and targets)
- Passive reference dye to maximize performance on different instrument platforms
- To improve results on multiple targets
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600804 |
Brilliant II QPCR Master Mix
Product Details
- Greater flexibility (numerous different templates and targets)
- Passive reference dye to maximize performance on different instrument platforms
- To improve results on multiple targets
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 400 Rxn | 600815 |
SYBR® Green
Are you planning to quantify a Next Generation Sequencing Library?
Yes
What types of NGS platform are you using?
Illumina
Recommended Product:
QPCR NGS Library Quant Kit, Illumina GA
Product Details
- Accurate library quantification
- Consistent library quantification across a broad range of samples
- Sensitivity of detection to quantify low concentration libraries
- Ideal for use in barcode sequencing
Ordering Info
| Size | Catalog |
|---|---|
| 1 Kit - 84 libraries | G4480A |
No
What is your preferred cycling format?
Fast
Recommended Product:
Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix
Product Details
- Reliable and reproducible data
- Novel fast Taq mutant (results in under 35 minutes)
- Enhanced rapid hot start capability
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600882 |
Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix
Product Details
- Reliable and reproducible data
- Novel fast Taq mutant (results in under 35 minutes)
- Enhanced rapid hot start capability
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 400 Rxn | 600883 |
Standard
Do you want ROX included in the Master Mix?
Yes
Do you want a Master Mix with High ROX (eg laser based instrument) or Low ROX (eg MX 3005P)?
High ROX
Recommended Product:
Brilliant II SYBR® Green High ROX QPCR Master Mix
Product Details
- Improved performance with earlier Ct detection
- Increased sensitivity of detection to low copy numbers
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600829 |
Low ROX
Recommended Product:
Brilliant II SYBR® Green Low ROX QPCR Master Mix
Product Details
- Improved performance with earlier Ct detection
- Increased sensitivity of detection to low copy numbers
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600830 |
No
Recommended Product:
Brilliant II SYBR® Green QPCR Master Mix
Product Details
- Earlier Ct detection
- Improved data reproducibility
- Greater flexibility for use with numerous different templates and targets
- More accurate quantification results
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600828 |
Brilliant II SYBR® Green QPCR Master Mix
Product Details
- Earlier Ct detection
- Improved data reproducibility
- Greater flexibility for use with numerous different templates and targets
- More accurate quantification results
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 400 Rxn | 600831 |
RNA
What kind of RNA are you working with?
Total/mRNA
Do you want 1 step or 2 steps?
1 Step
What chemistry will you be using?
Probes
What is your preferred cycling format?
Fast
Recommended Product:
Brilliant III Ultra-Fast QRT-PCR Master Mix
Product Details
- Extremely fast while maintaining sensitivity
- Greater resistance to common QPCR inhibitors
- Optimized fast cycling formulation
- Compatible with any sequence-specific probe detection chemistry
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600884 |
Brilliant III Ultra-Fast QRT-PCR Master Mix
Product Details
- Extremely fast while maintaining sensitivity
- Greater resistance to common QPCR inhibitors
- Optimized fast cycling formulation
- Compatible with any sequence-specific probe detection chemistry
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 400 Rxn | 600885 |
Standard
Do you want a Master Mix or an optimization kit?
Master Mix
Do you want a Master Mix with High ROX (eg laser based instrument) or Low ROX (eg MX 3005P)?
High ROX
Recommended Product:
Brilliant II QRT-PCR High ROX 1-Step Master Mix
Product Details
- Improved performance with earlier Ct detection
- Increased sensitivity of detection to low copy numbers
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600838 |
Low ROX
Recommended Product:
Brilliant II QRT-PCR Low ROX 1-Step Master Mix
Product Details
- Improved performance with earlier Ct detection
- Increased sensitivity of detection to low copy numbers
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600837 |
No ROX
Recommended Product:
Brilliant II QRT-PCR 1-Step Master Mix
Product Details
- Greater flexibility (numerous different templates and targets)
- Passive reference dye to maximize performance on different instrument platforms
- To improve results on multiple targets
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600809 |
Brilliant II QRT-PCR 1-Step Master Mix
Product Details
- Greater flexibility (numerous different templates and targets)
- Passive reference dye to maximize performance on different instrument platforms
- To improve results on multiple targets
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 400 Rxn | 600818 |
Optimization Kit
Recommended Product:
Brilliant II QRT-PCR 1-Step Core Kit
Product Details
- Greater flexibility (numerous different templates and targets)
- Passive reference dye to maximize performance on different instrument platforms
- To improve results on multiple targets
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600810 |
Brilliant II QRT-PCR 1-Step Core Kit
Product Details
- Greater flexibility (numerous different templates and targets)
- Passive reference dye to maximize performance on different instrument platforms
- To improve results on multiple targets
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 400 Rxn | 600819 |
SYBR® Green
What is your preferred cycling format?
Fast
Recommended Product:
Brilliant III Ultra-Fast SYBR® Green QRT-PCR Master Mix
Product Details
- Reliable and reproducible data
- Novel fast Taq mutant (results in under 35 minutes)
- Enhanced rapid hot start capability
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600886 |
Brilliant III Ultra-Fast SYBR® Green QRT-PCR Master Mix
Product Details
- Reliable and reproducible data
- Novel fast Taq mutant (results in under 35 minutes)
- Enhanced rapid hot start capability
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 400 Rxn | 600887 |
Standard
Do you want a Master Mix or an optimization kit?
Master Mix
Do you want a Master Mix with High ROX (eg laser based instrument) or Low ROX (eg MX 3005P)?
High ROX
Recommended Product:
Brilliant II Ultra-Fast SYBR® Green QRT-PCR Master Mix
Product Details
- Reliable and reproducible data
- Novel fast Taq mutant (results in under 35 minutes)
- Enhanced rapid hot start capability
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600836 |
Low ROX
Recommended Product:
Brilliant II QRT-PCR SYBR® Green Low ROX Master Mix
Product Details
- Improved performance with earlier Ct detection
- Increased sensitivity of detection to low copy numbers
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600835 |
No ROX
Recommended Product:
Brilliant II SYBR® Green QRT-PCR 1-Step Master Mix
Product Details
- Earlier Ct detection
- Improved data reproducibility
- Greater flexibility for use with numerous different templates and targets
- More accurate quantification results
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600825 |
Brilliant II SYBR® Green QRT-PCR 1-Step Master Mix
Product Details
- Earlier Ct detection
- Improved data reproducibility
- Greater flexibility for use with numerous different templates and targets
- More accurate quantification results
Ordering Info
| Size | Catalog |
|---|---|
| 10 x 400 Rxn | 600826 |
Optimization Kit
Recommended Product:
Brilliant II SYBR MM
Product Details
- Earlier Ct detection
- Improved data reproducibility
- Greater flexibility for use with numerous different templates and targets
- More accurate quantification results
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600825 |
Brilliant II SYBR MM
Product Details
- Earlier Ct detection
- Improved data reproducibility
- Greater flexibility for use with numerous different templates and targets
- More accurate quantification results
Ordering Info
| Size | Catalog |
|---|---|
| 4000 rxn | 600826 |
2 Steps
What chemistry will you be using?
Probes
What is your preferred cycling format?
Fast
Recommended Product:
Combination of Brilliant III QPCR MM + AffinityScript
Product Details
- Extremely fast while maintaining sensitivity
- Greater resistance to common QPCR inhibitors (i.e. whole blood or NaCl)
- Optimized fast cycling formulation with AffinityScript cDNA Synthesis Kit
- Fast, highly efficient cDNA
- Streamlined protocol produces cDNA in 15 minutes
- Linear detection from 3 pg to 3 µg total RNA
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600880 |
| + 50RXn | 600559 |
Combination of Brilliant III QPCR MM + AffinityScript
Product Details
- Extremely fast while maintaining sensitivity
- Greater resistance to common QPCR inhibitors (i.e. whole blood or NaCl)
- Optimized fast cycling formulation with AffinityScript cDNA Synthesis Kit
- Fast, highly efficient cDNA
- Streamlined protocol produces cDNA in 15 minutes
- Linear detection from 3 pg to 3 µg total RNA
Ordering Info
| Size | Catalog |
|---|---|
| 10 x400 RXn | 600881 |
| + 50RXn | 600559 |
Standard
Are you planning to multiplex with 3 or more targets?
Yes
Recommended Product:
Brilliant II SYBR® Green QRT-PCR AffinityScript 2-Step Master Mix
Product Details
- Available as a first-strand synthesis kit and in two-step QRT-PCR master mix formats
- Fast, highly efficient cDNA synthesis (produces cDNA in 15 minutes )
- Linear detection from 3 pg to 3 µg total RNA
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600834 |
| 50 Rxn | 600559 |
No
Do you want ROX included in your Master Mix?
Yes
No
Recommended Product:
Brilliant II QRT-PCR AffinityScript 2-Step Master Mix
Product Details
- Greater flexibility (numerous different templates and targets)
- Passive reference dye to maximize performance on different instrument platforms
- To improve results on multiple targets
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600827 |
Do you want a Master Mix with High ROX (eg laser based instrument) or Low ROX (eg MX 3005P)?
High ROX
Recommended Product:
Brilliant II qPCR High ROX Master Mix + cDNA synthesis kit
Product Details
- Formulations available with low and high concentrations of ROX reference dye
- Earlier Ct detection across a wide dynamic range
- Increased sensitivity of detection to low copy numbers
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600805 |
| 50 Rxn | 600559 |
Low ROX
Recommended Product:
Brilliant II qPCR Low ROX Master Mix + cDNA synthesis kit
Product Details
- Formulations available with low and high concentrations of ROX reference dye
- Earlier Ct detection across a wide dynamic range
- Increased sensitivity of detection to low copy numbers
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600806 |
| 50 Rxn | 600559 |
No
Recommended Product:
Brilliant II QRT-PCR AffinityScript 2-Step Master Mix
Product Details
- Greater flexibility (numerous different templates and targets)
- Passive reference dye to maximize performance on different instrument platforms
- To improve results on multiple targets
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600827 |
SYBR® Green
What is your preferred cycling format?
Fast
Recommended Product:
Combination of Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix + AffinityScript cDNA Synthesis Kit
Product Details
- Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix
- Reliable and reproducible data
- Novel fast Taq mutant for qPCR results in under 35 minutes
- Enhanced rapid hot start capability
- AffinityScript cDNA Synthesis Kit
- Fast, highly efficient cDNA
- Streamlined protocol produces cDNA in 15 minutes
- Linear detection from 3 pg to 3 µg total RNA
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600882 |
| + 50 Rxn | 600559 |
Combination of Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix + AffinityScript cDNA Synthesis Kit
Product Details
- Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix
- Reliable and reproducible data
- Novel fast Taq mutant for qPCR results in under 35 minutes
- Enhanced rapid hot start capability
- AffinityScript cDNA Synthesis Kit
- Fast, highly efficient cDNA
- Streamlined protocol produces cDNA in 15 minutes
- Linear detection from 3 pg to 3 µg total RNA
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600883 |
| + 50 Rxn | 600559 |
Standard
Recommended Product:
Brilliant II SYBR® Green QRT-PCR AffinityScript 2-Step Master Mix
Product Details
- Available as a first-strand synthesis kit and in two-step QRT-PCR master mix formats
- Fast, highly efficient cDNA synthesis (produces cDNA in 15 minutes)
- Linear detection from 3 pg to 3 µg total RNA
Ordering Info
| Size | Catalog |
|---|---|
| 400 Rxn | 600834 |
miRNA
Do you want to use a Master Mix or a Core Reagent Kit?
Master Mix
Recommended Product:
miRNA QPCR Master Mix
Product Details
- Detects mature miRNA
- Differentiate miRNA that differ by a single nucleotide
- Sensitive detection down to 10 copies
- Highly accurate results in 3 hours
- miRNA Specific Forward Primers
Ordering Info
| Size | Catalog |
|---|---|
| 200 Rxn | 600584 |
miRNA QPCR Master Mix
Product Details
- Detects mature miRNA
- Differentiate miRNA that differ by a single nucleotide
- Sensitive detection down to 10 copies
- Highly accurate results in 3 hours
- miRNA Specific Forward Primers
Ordering Info
| Size | Catalog |
|---|---|
| 200 Rxn | 600036 |
| 200 Rxn | 600583 |
Core Reagent Kit
Recommended Product:
miRNA QRT-PCR Master Mix Detection Kit
Product Details
- Detects mature miRNA
- Differentiate miRNA that differ by a single nucleotide
- Sensitive detection down to 10 copies
- Highly accurate results in 3 hours
- miRNA Specific Forward Primers
Ordering Info
| Size | Catalog |
|---|---|
| 200 Rxn | 600580 |
Brilliant II QRT-PCR SYBR® Green High ROX Master Mix
Product Details
- Improved performance with earlier Ct detection
- Increased sensitivity of detection to low copy numbers
Ordering Info
| Size | Catalog |
|---|---|
| 200 Rxn | 600036 |
| 200 Rxn | 600545 |
RNA Direct from Cells
Do you want 1 step or 2 steps?
1 Step
Recommended Product:
SideStep II QRT-PCR Master Mix
Product Details
- Quantification of RNA directly from cells without RNA purification
- Purifying only relevant samples after screening.
- RNA stabilized for up to 6 months
- Enables faster purification
- Excellent analysis flexibility
- RNA loss minimized
Ordering Info
| Size | Catalog |
|---|---|
| 200 Rxn | 400917 |
2 Steps
Recommended Product:
Combination of SideStep II Cell Analysis Kit + Brilliant III
Product Details
- Optimized cell lysis solution stabilizes RNA for at least 6 months
- Samples ready for QRT-PCR analysis in minutes with
- Brilliant III Ultra-Fast QRT-PCR Master Mix
- Extremely fast while maintaining sensitivity
- Greater resistance to common QPCR inhibitors (i.e. whole blood or NaCl)
Ordering Info
| Size | Catalog |
|---|---|
| 100 lysis | 400916 |
| + 400 Rxn | 600884 |
Combination of SideStep II Cell Analysis Kit + Brilliant III
Product Details
- Optimized cell lysis solution stabilizes RNA for at least 6 months
- Samples ready for QRT-PCR analysis in minutes with
- Brilliant III Ultra-Fast QRT-PCR Master Mix
- Extremely fast while maintaining sensitivity
- Greater resistance to common QPCR inhibitors (i.e. whole blood or NaCl)
Ordering Info
| Size | Catalog |
|---|---|
| 100 lysis | 400916 |
| + 10 x 400 Rxn | 600885 |
Welcome to the QPCR Decision Tree!
This decision tree is your personal guide through Agilent’s QPCR reagents portfolio. In just a few clicks, find out which QPCR solution best fits your application
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