| Description | |
|---|---|
| Catalogue Number | 14-486 |
| Brand Family | Upstate |
| Trade Name |
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| Description | p70 S6 Kinase Protein, active, 10 µg |
| Overview | N-terminal His6-tagged, recombinant amino acids 1-421 of human p70 S6 kinase |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged, recombinant amino acids 1-421 of human p70 S6 kinase, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Expressed in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. |
| Gene Symbol |
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| Protein Target | p70S6K |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | AGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P23443 # Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. SIZE: 525 amino acids; 59140 Da SUBUNIT: Interacts with PPP1R9A/neurabin-1 (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell junction, synapse, synaptosome (By similarity). TISSUE SPECIFICITY: Widely expressed. SIMILARITY: SwissProt: P23443 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain. |
| Molecular Weight | 48.7kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by phosphorylation of S6 Kinase/Rsk-2 Substrate Peptide 2 |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250μg size as catalog # 14-486M. |