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Millipore Pi 3-Kinase (P110alpha(H1047r)/P85alpha), 10 µG
Millipore Complex Of N-Terminal 6his-Tagged Recombinant Human P110α Full Length, Containing The Mutation H1047r & Untagged, Recombinant, Human P85α Full Length. - Mill (Additional S&H or Hazmat Fees May Apply)
Complex of N-terminal 6His-tagged recombinant human p110α full length, containing the mutation H1047R, and untagged, recombinant, human p85α full length.
Background Information
The H1047R substitution is a somatic mutation in p110α that has been associated with tumours of the colon, stomach, breast and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation. (Samuels Y. et al., Science, (2004); 304: 554; Kang S. et al., PNAS, (2005);102: 802-807 and Zhao J.J. et al., PNAS, (2005);102:18443-18448).
Product Information
Presentation
50 mM Tris/HCl pH8.0, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Quality Level
MQ100
Applications
Application
Complex of N-terminal 6His-tagged recombinant human p110α full length, containing the mutation H1047R & untagged, recombinant, human p85α full length.
Biological Information
Source
Co-expressed by baculovirus in Sf21 insect cells.
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
U79143
Gene Symbol
PIK3CA
PI3K
PK3CA
Protein Target
PI3Kα/p85α
Purification Method
Ni2+/NTA-agarose
Target Sub-Family
PI3K/PI4K
UniProt Number
P42336 / P27986
P42337 / P26450 (mouse)
Molecular Weight
p110α(H1047R) MW = 125 kDa, p85α MW = 84 kDa.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Packaging Information
Material Size
10 µg
Material Package
Also available in 250μg size --call for pricing and availability and reference catalog number 14-792M when ordering the 250μg size.
