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Millipore Target-Specific Spike-In Controls That Make Chip Experiments More Quantitative And Accurate. Applications Include: Normalization Of Chip Signals Normalization Of Chip-Seq - Mill (Additional S&H or Hazmat Fees May Apply)
AccuChIP™ Trimethyl-Histone H3 (Lys4) Chromatin Immunoprecipitation Internal Control
Overview
AccuChIP™ Chromatin Immunoprecipitation Internal Controls are designed to improve the accuracy of ChIP experiments. This simple to spike-in reagent is composed of a target-specific peptide sequence covalently bound to a uniquely designed double stranded nucleic acid sequence. Because AccuChIP controls mimic the behavior of cross-linked chromatin, by simply adding a known amount of the AccuChIP reagent to your chromatin and performing a standard ChIP reaction followed qPCR detection you can improve the accuracy and reproducibility of your ChIP experiments.
Features and Advantages
Simple to use - just add to chromatin either before or after sonication
Mimics the behavior of cross-linked chromatin to allow normalization of ChIP signals
Target specific control - uses the same antibody used for your ChIP experiment
Reduces ChIP variation due to variables such as chromatin preparation, antibody affinity, inconsistent background signals from carry over, and loss of material during handling
Easily incorporated into virtually any ChIP protocol
Readily detected by qPCR
Easily incorporated into ChIP-seq libraries
Variety of uniquely designed controls available -add multiple controls to be added to the same chromatin as an process antibody specificity
Materials Required but Not Delivered
Reagents and materials for chromatin isolation from cells or tissues (e.g PureEpi Chromatin Preparation and Optimization kit; EMD Millipore Cat# 17-10082)
Sonication device or method to shear chromatin
Beads, reagents and buffers to perform chromatin immunoprecipitation (e.g. Magna ChIP HiSens Chromatin Immunoprecipitation Kit EMD Millipore Cat# 17-10460, or EZ-Magna ChIP HiSens Chromatin Immunoprecipitation Kit Cat# 17-10461)
ChIP validated antibody or ChIPAb+™ kit
IgG negative control antibody (visit www.emdmillipore.com/antibodies to order the IgG negative control antibody used with this control)
Real time thermal cycler and qPCR reagents and primers for locus of interest
Reagents and instrument for next generation sequencing analysis (optional)
Product Information
Components
AccuChIP™ H3K4me3 Spike-In Reagent (50 pg/uL )
AccuChIP™ H3K4me3 Detection Primer Mix (5 μM)
AccuChIP™ Dilution Buffer
Presentation
One box containing three vials (one vial each of target specific AccuChIP™ Spike-In Reagent, target specific AccuChIP™ Detection Primer Mix, and AccuChIP™ Dilution Buffer)
Quality Level
MQ100
Applications
Application
Target-specific spike-in controls that make ChIP experiments more quantitative and accurate. Applications include:
Normalization of ChIP signals
Normalization of ChIP-seq signals
Analysis of antibody specificity
Key Applications
Chromatin Immunoprecipitation (ChIP)
ChIP-seq
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
AccuChIP™ Chromatin Immunoprecipitation Internal Controls are stable for 6 months at -20 ºC from date of receipt. AccuChIP Spike In Reagent is provided as a 20X stock and should be diluted with the provided AccuChIP™ Dilution Buffer to create a working stock. Diluted AccuChIP Spike In Reagent can be stored in aliquots at -20 ºC for up to 3 months. AccuChIP Spike In reagent is composed of a peptide-double stranded DNA conjugate in a buffer containing BSA and glycerol to reduce adherence to the tube. The use of siliconized microfuge tubes for storage of diluted material is recommended.
