Agilent Technologies Xl1-Blue Mrf? Electroporation-Competent Cells

Agilent Technologies Xl1-Blue Mrf’ Electroporation-Competent Cells; Restriction Minus For Cloning Methylated Dna, ? 1 X 1010 Transformants/Ug - Xl1-Blue Mr & Mrf Competent Cells
Cleavage Of Dna Before Host Replication Creates Libraries That Lack Complete Representation.
Xl2-Blue Mrf? Ultracompetent Cells Are High-Efficiency Derivatives Of Xl1-Blue Mrf? Supercompetent Cellsxl1-Blue Mrf’ Cells Are Deficient In All Known Restriction Systems And Are Are Endonuclease (Enda), And Recombination (Reca) Deficientxl1-Blue Mr Cells Are Restriction Minus (Mcra–, Mcrcb–, Mcrf–, Mrr, Hsdr–) Derivative Of Our Xl1-Blue Strain And Is Useful For Cosmid-Based Cloning
If Dna Is Methylated In A Fashion Unlike The Bacterial Host Patterns, It Is Cleaved By The E. Coli Host Restriction Systems.
Mr (Minus Restriction) Series Of Competent Cells Are Deficient In All Known E. Coli K12 Restriction Systems And Make It Possible To Clone Methylated Dna, Which Can Be Very Useful In Epigenetic Studies Where Dna Methylation, Instead Of Changes In Dna Sequence, May Be Associated With Heritable Changes In Gene Expression Or Cellular Phenotype.
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