We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Share this :
Print
Cell Signaling Atg13 (E1y9v) Rabbit mAb
Cell Signaling Atg13 (E1y9v) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$139.00
Your Price
$139.00
HOW MUCH YOU SAVE:
0.00 %
NETA PART: | CSIG-13468T |
MFG.PART: | 13468T |
UNSPSC: | 41116133 |
Manufacturer: | Cell Signaling |
Size | 20 µl |
Reactivity | H |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 72 |
Source/Isotype | Rabbit IgG |
Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunofluorescence (Immunocytochemistry): 1:100 - 1:200} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | Atg13 (E1Y9V) Rabbit mAb recognizes endogenous levels of total Atg13 protein. |
Species Reactivity | Human |
Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn230 of human Atg13 protein. |
Background | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes.Atg13/Apg13 was originally identified in yeast as a constitutively expressed protein that was genetically linked to Atg1/Apg1, a protein kinase required for autophagy (4). Overexpression of Atg1 suppresses the defects in autophagy observed in Atg13 mutants (4). Autophagy requires a direct association between Atg1 and Atg13, and is inhibited by TOR-dependent phosphorylation of Atg13 under high-nutrient conditions (5). Similarly, mammalian Atg13 forms a complex with the Atg1 homologues ULK1/2, along with FIP200, which localizes to autophagic isolation membranes and regulates autophagosome biogenesis (6-8). mTOR phosphorylates both Atg13 and ULK1, suppressing ULK1 kinase activity and autophagy (7-9). ULK1 can directly phosphorylate Atg13 at a yet unidentified site, presumably to promote autophagy (7,8). Additional studies suggest that Atg13 and FIP200 can function independently of ULK1 and ULK2 to induce autophagy through an unknown mechanism (10). |
SKU | CSIG-13468T |
---|---|
Supplier Part Number | 13468T |
UM | UNIT |
UNSPSC | 41116133 |
Manufacturer Name | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |