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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Xn, F, Sh, Dr, B, R, M | ICC | M | AlexaFluor®555 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1618-AF555 |
| Description | Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 555 conjugate |
| Alternate Names |
|
| Background Information | Cytoplasmic dynein 1 intermediate chain 1 (UniProt Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) and Cytoplasmic dynein 1 intermediate chain 2 (UniProt Q0III3; also known as Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosolic, DH IC-2) are encoded by the DYNC1I1 gene (Gene ID 613724) and DYNC1I2 gene (Gene ID 526329) in bovine, respectively. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ∼1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 & IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus. |
| Product Information | |
|---|---|
| Format | AlexaFluor®555 |
| Presentation | Purified mouse monoclonal IgG2b antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Dynein, IC, cytosolic Antibody, clone 74.1, Alexa Fluor® 555 conjugate is an antibody against Dynein for use in Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution of this antibody from a representative lot detected Dynein in A431, HUVECs, and NIH/3T3 cells. The unconjugated antibody (Cat. No. MAB1618) is shown to be suitable also for Western blotting, immunocytochemistry, immunofluorescence, and immunoprecipitation applications. |
| Biological Information | |
|---|---|
| Immunogen | Bovine brain cytoplasmic dynein. |
| Epitope | Within N-terminal 60 amino acids. |
| Clone | 74.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Reacts with all known forms of the Mr = 74,000 Dalton intermediate chain subunit (IC74) of mammaliam cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes). |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| Molecular Weight | ~74 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Dynein in HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |