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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, Mplex | Rb | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE643 |
| Description | Anti-monomethyl Histone H3 (Lys79), clone RM147 Antibody |
| Alternate Names |
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| Background Information | Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Lysine methylation occurs in three distinct states, having either one (me1), two (me2) or three (me3) methyl groups attached to the amine group of the lysine side chain. Methylation of Lysine 79 in Histone 3 acts as a marker of inactive chromatin regions. Methylation by Dot1 methylase on lysine-79 (Lys79) of Histone H3 is criticial for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-monomethyl Histone H3 (Lys79), clone RM147 Antibody is validated for use in Western Blotting, Multiplexing for the detection of monomethyl Histone H3. |
| Key Applications |
|
| Application Notes | Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex. |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to human Histone H3 monomethylated at Lys79. |
| Clone | RM147 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This antibody reacts to Histone H3 monomethylated at Ly79 (K79me1). No cross reactivity with dimethylated Lys79 (K79me2) or trimethylated Lys79 (K79me3), or other methylations in histone H3. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa acid extract and HeLa cell lysate. Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected monomethyl Histone H3 (Lys79) in HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |