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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC | AHm | Affinity Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF1579 |
| Description | Anti-CD3e (Mouse), clone 145-2C11, biotin conjugate Antibody |
| Alternate Names |
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| Background Information | The 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The 145-2C11 antibody is a widely used phenotypic marker for mouse T cells. In addition, binding of 145-2C11 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified Armenian Hamster monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD3e (Mouse), clone 145-2C11, biotin conjugate Antibody detects level of CD3e and has been published and validated for use in Flow Cytometry. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Immunogen information for this antibody is proprietary information. |
| Clone | 145-2C11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Armenian Hamster |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Purified by size-exclusion chromatography |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD3e in one million C57Bl/6 splenocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |