Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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H | IH(P) | M | Purified | Monoclonal Antibody |
Description | |
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Catalogue Number | MABS1922 |
Description | Anti-Src-Like-Adaptor 2 (SLA2) Antibody, clone Z30P1F12*F4 |
Alternate Names |
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Background Information | Src-like-adapter 2 (UniProt: Q9H6Q3; also known as Modulator of antigen receptor signaling; MARS; Src-like adapter protein 2; SLAP-2) is encoded by the SLA2 (also known as SLAP2, C20orf156) gene (Gene ID 84174) in human. SLAP-2 is localized to the plasma membrane and intracellular vesicles, including late endosomal vesicles. The SLAP-2 protein contains an NH2-terminal myristoylation consensus sequence and SH3 and SH2 Src homology domains, but lacks a tyrosine kinase domain. It is predominantly expressed in immune system, including T cells and monocytes and at low levels in B cells, placenta, prostate, colon, and skin. SLAP-2 serves as an adapter protein that negatively regulates T-cell receptor (TCR) signaling and is shown to block T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. In antigen receptor-stimulated cells, SLAP-2 associates with several tyrosine phosphorylated proteins, including the ubiquitin ligase Cbl. Deletion of the carboxyl terminus of SLAP-2 is shown to block its function and abolish its association with Cbl. Overexpression of SLAP-2 in T and B cell lines impairs antigen receptor-mediated signaling events, including the up-regulation of CD69, NFAT promoter activation, and calcium influx. Ref.: Holland, SJ et al. (2001). J. Exp. Med. 194(9):1263-1276. Pandey, A., et al. (2002). J. Biol. Chem. 277(21):19131-19138. |
Product Information | |
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Format | Purified |
Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
Quality Level | MQ100 |
Applications | |
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Application | Detect Src-like-adapter 2 using this mouse monoclonal Anti-Src-Like-Adaptor 2 (SLA2) antibody, clone Z30P1F12*F4, Cat. No. MABS1922, validated for use in Immunohistochemistry (Paraffin). |
Key Applications |
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Biological Information | |
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Immunogen | A synthetic peptide corresponding to 8 aa from the C-terminal region of human Src-like adapter 2 protein. |
Clone | Z30P1F12*F4 |
Concentration | Please refer to lot specific datasheet. |
Host | Mouse |
Specificity | Clone Z30P1F12*F4 detects -Src-Like-Adaptor 2 (SLA2) protein in human by targeting a sequence in the C-terminal region. |
Isotype | IgG1κ |
Species Reactivity |
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Species Reactivity Note | Human. |
Antibody Type | Monoclonal Antibody |
Entrez Gene Number |
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Gene Symbol |
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Purification Method | Protein G purified |
UniProt Number |
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Molecular Weight | 28.6 kDa calculated. |
Product Usage Statements | |
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Quality Assurance | Evaluated by Immunohistochemistry in human cervical cancer, pancreas, and tonsil tissue. Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Src-Like-Adaptor 2 (SLA2) in human cervical cancer, pancreas, and tonsil tissue. |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
Packaging Information | |
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Material Size | 100 μg |