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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS497 |
| Description | Anti-Cytochrome P450 26B1 Antibody, clone 4B7.2 |
| Alternate Names |
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| Background Information | Cytochrome P450 26B1 (UniProt Q9NR63; also known as Cytochrome P450 26A2, Cytochrome P450 retinoic acid-inactivating 2, Cytochrome P450, subfamily XXVIB, polypeptide 1, Cytochrome P450RAI-2, Retinoic acid-metabolizing cytochrome) is encoded by the CYP26B1 (also known as CYP26A2, P450RAI2, RHFCA) gene (Gene ID 56603) in human. Cytochromes P450 (CYP) proteins are primarily membrane-associated oxidases located either in the inner membrane of mitochondria or in the endoplasmic reticulum. In addition to processing endogenous substrates, CYPs also metabolize exogenous drugs and potentially toxic chemicals. The human CYP superfamily consists of 57 genes and more than 59 pseudogenes divided into 18 families and 43 subfamilies. The CYP26 subfamily of enzymes (CYP26A1, CYP26B1/CYP26A2, CYP26C1) are retinoic acid hydroxylases responsible for the inactivation of all-trans-retinoic acid to hydroxylated forms, such as 4-oxo-, 4-OH-, and 18-OH-all-trans-retinoic acid. CYP26B1 is overexpressed in colorectal cancer (CRC) cells, high CYP26B1 expression level in CRC patients is associated with poor prognosis. CYP26B1 mutations are found in radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA), a disease characterized by craniofacial malformations, occipital encephalocele, radiohumeral fusions, oligodactyly, advanced osseous maturation, and calvarial mineralization defects. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cytochrome P450 26B1 Antibody, clone 4B7.2 is an antibody against Cytochrome P450 26B1 for use in Western Blotting, Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cytochrome P450 26B1 in human brain, mouse brain, and rat cerebellum tissue. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant internal fragment from the C-terminal half of human Cytochrome P450 26B1. |
| Epitope | Internal (C-terminal half). |
| Clone | 4B7.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with all three spliced isoforms of human CYP26B1 reported by UniProt (Q9NR63). |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~57 kDa observed. 57.51 kDa (isoform 1), 49.01 kDa (isoform 2), and 55.76 kDa (isoform 3) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue microsomal lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected Cytochrome P450 26B1 in 10 µg of mouse brain tissue microsomal lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |