LICOR GAR/GAM – CellTag 520 ICW Kit IV - LIC-926-42094

LI-COR Biosciences GAR/GAM CellTag 520 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays. This cost-effective normalization method makes quantification of the target protein more precise. CellTag 520 Stain accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number.

The In-Cell Western (ICW) Assay is a quantitative immunofluorescence assay performed in microplates (optimized for 96- or 384-well format) that combines the specificity of Western blotting with the replicability and throughput of ELISA.

In-Cell Western Assays are also called cytoblots, cell-based ELISA, In-Cell ELISA (ICE), and FACE (Fast Activated Cell-based ELISA). With In-Cell Western Assays, you can:

• Detect proteins in fixed and permeabilized cultured cells using target-specific primary antibodies and IRDye^® secondary antibodies
• Quantify two targets at 700 nm and 800 nm, using spectrally distinct infrared dye conjugates
• Quickly, accurately measure relative protein levels in many samples
• Detect proteins in situ in a relevant cellular context
• Normalize to cell number, allowing for accurate quantification and comparison of protein expression between wells
  
  

This kit includes reagents to perform an In-Cell Western Assay and CellTag 520 Stain to normalize well-to-well variations in cell number.

Kit Components

• IRDye^® 800CW Goat anti-Rabbit Secondary Antibody, 0.5 mg
• IRDye 680RD Goat anti-Mouse Secondary Antibody, 0.5 mg
• Intercept^® (TBS) Blocking Buffer, 1 x 500 mL
• CellTag 520 Stain, 2 x 50 nmol
• Kit Protocol