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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M | WB, ICC, IF, IH(P) | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-1565 |
| Description | Anti-NSD1 Antibody, clone 1NW-1A10 |
| Alternate Names |
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| Background Information | NSD1 (also known as KMT3B) is a histone methyltransferase shown to methylate lysine 36 of histone H3 and lysine 20 of histone H4. NSD1 is a described as an intermediary transcription factor and depending on its cellular context, it is able to either negatively or positively influence transcription. Expression of this gene has been described in skeletal muscles, kidneys, adult and fetal brain, thymus and spleen. Heterozygous mutations and deletions of the NSD1 gene are associated with Sotos Syndrome, an autosomal dominant condition characterized by macrocephaly, tall stature, facial elongation, accelerated bone aging, and mental retardation. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
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| Presentation | Unpurified mouse monoclonal IgG1κ in preservative-free ascites. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NSD1 Antibody, clone 1NW-1A10 is a mouse monoclonal antibody for detection of NSD1, also known as &rogen receptor-associated coregulator 267, Lysine N-methyltransferase 3B, has been validated in WB. |
| Key Applications |
|
| Application Notes | Used for immunohistochemistry on paraffin sections in Visser et al., (2012). Used for fluorescent immunocytochemistry in Huang et al.,(1998). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to mouse NSD1. |
| Epitope | Unknown |
| Clone | clone 1NW-1A10 |
| Host | Mouse |
| Specificity | This antibody recognizes NSD1. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with rat. Predicted to react with mouse based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Histone methyltransferase. Preferentially methylates Lys-36 of histone H3 and Lys-20 of histone H4 (in vitro). Transcriptional intermediary factor capable of negatively influencing transcription. May also positively influence transcription. Essential for early post-implantation development. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. SUBUNIT STRUCTURE: Interacts with AR DNA- and ligand-binding domains by similarity. Interacts with the ligand-binding domains of RARA and THRA in the absence of ligand; in the presence of ligand the interaction is severely disrupted but some binding still occurs. Interacts with the ligand-binding domains of RXRA and ESRRA only in the presence of ligand. SUBCELLULAR LOCATION: Nucleus TISSUE SPECIFICITY: Expressed in the embryo and the outer region of the uterine decidua at early post-implantation E5.5 stage. Uniformly expressed in embryonic and extraembryonic tissues during gastrulation stage E7.5. Expressed differentially after stage 14.5 with highest expression in proliferating cells. Enriched in the telencephalic region of the brain, spinal cord, intestinal crypt, tooth buds, thymus and salivary glands at stage E16.5. Also expressed in the ossification region of developing bones and in the periosteum. DOMAIN: Contains 2 adjacent but distinct nuclear receptor interaction domains (NID+L and NID-L) to which nuclear receptors bind differentially in the presence and absence of ligand respectively. SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family. Contains 1 AWS domain. Contains 4 PHD-type zinc fingers. Contains 1 post-SET domain. Contains 1 PWWP domain. Contains 1 SET domain. |
| Molecular Weight | ~ 300 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in PC12 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected NSD1 on 10 µg of PC12 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |