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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB, ICC, ELISA, IHC, IF, FC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1050 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | 4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b) |
| Background Information | The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Proprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2b) and PY20 (IgG2b) in 0.1 M Tris-glycine, Ph 7.4, 0.15 M NaCl, 0.05% sodium azide. Liquid at 2-8°C. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | 4G10 Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) detects levels of Phosphotyrosine proteins & has been published & validated for use in WB, ICC, ELISA, IHC, IF, Flow & IP. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: 2-4 μL of a previous lot can immunoprecipitate quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer. |
| Biological Information | |
|---|---|
| Immunogen | 4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein |
| Clone | 4G10® |
| Host | Mouse |
| Specificity | Tyrosine-phosphorylated proteins from all species |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Modifications |
|
| Purification Method | Protein G-Sepharose™ Chromatography |
| Molecular Weight | Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells. Western Blot Analysis: 1:1,000-1:2,000 of this antibody detected tyrosinephosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |