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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H | ICC, IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-923 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Cdk1/Cdc2 (PSTAIR) Antibody |
| Background Information | Cyclin-dependent kinase 1 (UniProt: P06493; also known as EC:2.7.11.22, EC:2.7.11.23), CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase) is encoded by the CDK1 ( also known as CDC2, CDC28A, CDKN1, P34CDC2) gene (Gene ID: 983) in human. CDK1 is an archetypical kinase that forms a stable, but non-covalent complex with a regulatory subunit and with a cyclin. It interacts with cyclins-B to form a serine/threonine kinase holoenzyme complex known as the maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. It can also form CDK1-cylin-D and CDK1-cyclin-E complexes that phosphorylate RB1 in vitro. Via these interactions it plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset. It promotes G2-M transition and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. CDK1 is mostly cytoplasmic during the interphase and is reversibly translocated from cytoplasm to nucleus when phosphorylated before the G2-M transition. It can undergo phosphorylation at multiple sites and each phosphorylation has a specific effect. Phosphorylation at Thr 161 by CAK/CDK7 is reported to activate its kinase activity and phosphorylation at Thr 14 and Tyr 15 by PYMYT1, a member of the WEE kinase family, prevents its nuclear translocation and inactivates this enzyme. Phosphorylation at Tyr 15 by WEE1 and WEE2 inhibits its protein kinase activity and blocks its entry into mitosis. This keeps CDK1-Cyclin B complex in an inactive state until the end of G2 phase. By the end of G2 phase, PKMYT1 and WEE1 are inactivated and CDC25A and CDC25B are activated, which dephosphorylate Thr 14 and Tyr 15 leading to CDK1 activation. (Ref.: Diril, MK., et al., (2012). Proc. Natl. Acad. Sci. USA. 109(10); 3826-3831; Draetta, G., and Beach, D. (1988). Cell. 54(1); 17-26). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cdk1/cdc2 (PSTAIR), Cat. No. 06-923, is a rabbit polyclonal antibody that detects Cdk1/Cdc2 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested applications Western Blotting Analysis: A 1: 10,000 dilution from a representative lot detected Cdk1/Cdc2 (PSTAIR) in NIH 3T3 and L6 cell lysates. Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Cdk1/Cdc2 (PSTAIR) in A431 cells. Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated Cdk1/Cdc2 in A431 cell lysate. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Cyclin-dependent kinase 1 (Cdk1). |
| Epitope | N-terminus |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Cdk1 in multiple species. It targets an epitope within 16 amino acids from the N-terminal region. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Amphibians, Chicken, Canine, Drosophila, Monkey, Sheep based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. |
| Gene Symbol |
|
| Purification Method | Antigen Affinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. SIZE: 297 amino acids; 34095 Da SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase. SUBCELLULAR LOCATION: Nucleus (By similarity). SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | ~34 kDa observed; 34.09 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431cell lysate. Western Blotting Analysis: A 1: 10,000 dilution of this antibody detected Cdk1/Cdc2 (PSTAIR) in A431cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |