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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-315 |
| Replaces | 04-1027 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-SGK1 Antibody |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Immunoaffinity Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and 1.75mg/ml BSA before the addition of glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-SGK1 Antibody is validated for use in IP, WB for the detection of SGK1. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | peptide corresponding to amino acids 399-412 (C-GKSPDSVLVTASVK) of human SGK1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes SGK1. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a serine/threonine protein kinase that is highly similar to the rat serum-and glucocorticoid-induced protein kinase (SGK). This gene was identified in a screen of hepatocellular genes regulated in response to cellular hydration or swelling. Cellular hydration is a catabolic signal, stimulating glycogenolysis and proteolysis, and inhibiting protein and glycogen synthesis. This kinase has been shown to be important in activating certain potassium, sodium, and chloride channels. Expression of this gene in hepatocytes is stimulated by transforming growth factor-beta (TGF-beta) which participates in the pathophysiology of diabetic complications. Since both TGF-beta expression and SGK expression are elevated in diabetic nephropathy, this suggests an involvement of SGK in the development of this condition. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O00141 # Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1. SIZE: 431 amino acids; 48942 Da SUBUNIT: Interacts with NEDD4 and NEDD4L. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Endoplasmic reticulum. Note=Nuclear, upon phosphorylation. TISSUE SPECIFICITY: Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. PTM: Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3- kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. & Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. SIMILARITY: SwissProt: O00141 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain. |
| Molecular Weight | ~50 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from A431 and Rat 2 cells |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |