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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, Ht | WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10533 |
| Description | Anti-LMX-1 Antibody |
| Alternate Names |
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| Background Information | LMX-1 is a transcription factor that belongs to the LIM-homeodomain (LIM-HD) transcription factor family. It is induced within the ventral midbrain as a response to early signaling. LMX-1 is a necessary component for the development of the roof plate and for CNS dorsal cell date specification and the development of vertebrae. It is Expressed in roof plate, an organizational locus found in the dorsal CNS that functions to moderate the differentiation and specification of adjacent neurons through the secretion of signaling molecules such as WNT and BMP. Recent studies have indicated that LMX-1 has high potential for stimulating the production of mesencephalic dopamine neurons(mesDA) for the therapeutic treatment Parkinson disease, a condition characterized by dopaminergic neurotransmission decline resulting from the progression of mesDA neuronal degeneration within the substantia nigra pars compacta (SN). |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Unpurified rabbit polyclonal serum containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LMX-1 Antibody is an antibody against LMX-1 for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the C-terminus of hamster LMX-1. |
| Epitope | C-terminus |
| Host | Rabbit |
| Specificity | This antibody recognizes LMX-1. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with mouse and rat. Predicted to react with hamster based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae (By similarity). SUBCELLULAR LOCATION: Nucleus. SEQUENCE SIMILARITIES: Contains 1 homeobox DNA-binding domain. Contains 2 LIM zinc-binding domains. |
| Molecular Weight | ~50 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse testis tissue lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected LMX-1 on 10 µg of mouse testis tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |