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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | ELISA, IHC, IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1591P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Dopamine Transporter Antibody |
| Alternate Names |
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| Background Information | Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinsons disease, Tourettes disease, schizophrenia, and addiction. It is a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Affinity Purified immunoglobulin. Liquid in PBS containing 0.1% BSA |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Dopamine Transporter using this Anti-Dopamine Transporter Antibody validated for use in ELISA, IH, IP & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL using ECL. An antibody raised against a similar peptide has been shown to react with a broad 75-80 kDa band in rat striatum (Hastrup et al., 2001) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa. Immunoprecipitation: 2-10 μg of antibody (Chang et al., 2001) Immunohistochemistry: 1:250-1:500 on frozen sections of tissues fixed with 4% paraformaldehyde (Chang et al., 2001; Ourednik, et al., 2002). 0.05% trypsin for 10-15min has been found effective in exposing the epitope in some preparations. ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide (Catalog Number AG296) per well. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (Kilty et al. 1991; Giros et al. 1991; Shimada et al. 1991; Kitayma 1992). This region is presumably the cytoplasmic domain. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes Dopamine transporter (DAT). By Western blot the antibody recognizes a broad 80 kDa band in rat striatum (Lew et al. 1992; Freed et al. 1995; Vaughan et al. 1996) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The dopamine transporter (DAT1) mediates the active reuptake of dopamine from the synapse and is a principal regulator of dopaminergic neurotransmission. The DAT1 gene has been implicated in human disorders such as parkinsonism, Tourette syndrome, and substance abuse (Vandenbergh et al., 1992 [PubMed 1359373]).[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q01959 # Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. SIZE: 620 amino acids; 68495 Da SUBUNIT: Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: Q01959 ## Belongs to the sodium:neurotransmitter symporter (SNF) family. MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine. |
| Molecular Weight | 80 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |