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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1787P |
| Replaces | AB1789P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Dopamine D4 Receptor Antibody, extracellular domain |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Dopamine D4 Receptor Antibody, extracellular domain is an antibody against Dopamine D4 Receptor for use in ELISA & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL (Chemiluminescence technique). Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band. Antibody detects ~55kDa band in HT222 cells and mouse hippocampal membranes. {http://www.jneurosci.org/cgi/reprint/21/16/6069.pdf, Ishige, K et.al.(2001) J. Neuroscience 21(16):6069-6076. ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide/well) Not tested in immunohistochemical methods. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 10 amino acid peptide sequence from the mouse D4 receptor within the 3rd extracellular domain. |
| Epitope | extracellular domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Specific for Dopamine Receptor D4. Mouse Dopamine receptor D4 is a 387 aa, G-protein coupled and transmembrane receptor protein (Van Tol et al. 1992; Legerski & Peterson 1992; Livingstone et al. 1992; Seeman et al. 1994). The immunogen peptide shows no significant homology with other dopamine receptors (D1, D2, D3 or D5). SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 90% with rat and human D4 receptors (Van Tol et al. 1992; Legerski & Peterson 1992; Livingstone et al. 1992; Seeman et al. 1994; OMalley et al. 1992). Antibody cross-reactivity with D4R from various species is not known. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P21917 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. SIZE: 467 amino acids; 48361 Da SUBUNIT: Interacts with CLIC6 (By similarity) and GPRASP1. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P21917 ## Belongs to the G-protein coupled receptor 1 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |