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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB | Gt | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB742 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Apolipoprotein B Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Defibrinated, delipidized and absorbed by solid phase chromatography as required. Goat antisera in 0.05M Tris-HCl pH 7.5, 0.5M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Apolipoprotein B Antibody is an antibody against Apolipoprotein B for use in IP & WB. |
| Key Applications |
|
| Application Notes | Western Blot Immunoprecipitation Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified human Apolipoprotein B. |
| Host | Goat |
| Specificity | Apolipoprotein B. Monospecific by IEP with neat antibody vs. 2X pooled human serum and neat pooled human plasma. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P04114 # Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. SIZE: 4563 amino acids; 515563 Da SUBCELLULAR LOCATION: Secreted. PTM: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. DISEASE: SwissProt: P04114 # Defects in APOB are a cause of familial hypobetalipoproteinemia (FHBL) [MIM:107730]. FHBL is a genetically heterogeneous autosomal co-dominant disorder, associated with reduced plasma concentrations of apoB, LDL and VLDL. Heterozygotes for FHBL are usually asymptomatic with LDL cholesterol and apoB- 100 concentrations less than 50% of those in normal plasma. Homozygotes have extremely low plasma LDL cholesterol and apoB-100 concentrations, and clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia [MIM:200100]. & Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB) [MIM:144010]. FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. & Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.SIMILARITY:SwissProt: P04114 ## Contains 1 vitellogenin domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 1 mL |