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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Mk | FC, IP, IHC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL496 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-CD34 Class II Antibody, clone QBEND/10 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD34 Antibody, Class II, clone QBEND/10 is an antibody against CD34 Class II for use in FC, IP, IH, IH(P). |
| Key Applications |
|
| Application Notes | Identification of haemopoietic progenitor cells Studies into the control mechanisms of early haemopoiesis Purification of haemopoietic cells from, for example, FACS The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts Immunohistochemistry in parraffin sections: Pretreatment is HIER with citrate buffer, pH 6.0. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Human endothelial vesicles |
| Clone | QBEND/10 |
| Host | Mouse |
| Specificity | The antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. The antigen is expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. This antibody recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage. KNOWN SPECIES CROSS REACTIVITY: Positive with rhesus monkey peripheral blood leucocytes in flow cytometry. FUSION PARTNER: NSO myeloma cell line |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM] |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P28906 # Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. SIZE: 385 amino acids; 40716 Da SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells. PTM: Highly glycosylated. & Phosphorylated on serine residues by PKC. DISEASE:SwissProt: P28906 # Abnormal CD34 expression in leukemogenesis. SIMILARITY: SwissProt: P28906 ## Belongs to the CD34 family. |
| Product Usage Statements | |
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| Availability by Geography |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |