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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | Phycoerythrin | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | FCMAB214P |
| Trade Name |
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| Description | Milli-Mark™ Anti-CD117 Antibody, c-kit-PE, clone 104D2 |
| Alternate Names |
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| Background Information | CD117 is a 145 kDa transmembrane protein which belongs to the class III receptor tyrosine kinase family. The extracellular region consists of five immunoglobulin-like domains where the second and third loops are thought to be involved in ligand binding (Ashman, 1997; Bühring, 1995). CD117 has been shown to be a marker for tissue mast cells, hematopoietic stem cells, and progenitor cells in normal human bone marrow. The majority of CD117-positive marrow cells (50-70%) co-express CD34 and comprise progenitor cells and their precursors of all hematopoietic lineages. Apart from mast cells, CD117 is essentially absent from mature hematopoietic cells. Although CD117 is primarily a marker for non-lymphoid progenitor cells in the hematopoietic cells/tissues, including melanocytes, embryonic brain, and the reproductive system (Ashman, 1997). Anti-CD117, 104D2, was included in the Sixth International Workshop and Conference on Human Leucocyte differentiation Antigens, and studies by different laboratories confirmed its reactivity with CD117 (Ashman, 1997). |
| Product Information | |
|---|---|
| Format | Phycoerythrin |
| Control |
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| Presentation | Purified Mouse monoclonal IgG1 conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Milli-Mark Anti-CD117 Antibody, c-kit-PE, clone 104D2 detects level of CD117 & has been published & validated for use in FC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | MOLM-1 megakaryocytic cell line. |
| Clone | 104D2 |
| Host | Mouse |
| Specificity | Antibody recognizes Human CD117 antigen. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (PI3K). Defects in KIT are a cause of piebaldism. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes |
| Molecular Weight | 145 kDa (Ashman, 1997; Buhring, 1995) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by flow cytometry using TF-1 cells |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 tests |