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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3069 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Connexin 32 Antibody, clone M12.13 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in 0.02M PB, 0.25M NaCl, containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Connexin 32 Antibody, clone M12.13 detects level of Connexin 32 & has been published & validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: acetone or unfixed tissues recommended. Epitope is internal, paraffin sections are untested. Works with lightly formaldehyde-fixed tissue. Western blot: Detects a 27 kDa Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47 kDa band corresponding to aggregates. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Clone | M12.13 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Monoclonal antibody MAB3069 was produced by the immunization of BALB/c mice with isolated rat junctional complexes, prepared by the method of Stevenson and Goodenough (1988). Epitope mapping, hydropathy plotting, and topological studies suggest that this antibody is very likely directed against an epitope located between residues 95-125 in the central cytoplasmic loop of rat Cx32. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells (Bergoffen et al., 1993 [PubMed 8266101]). For a general discussion of connexin proteins, see GJB2 (MIM 121011).[supplied by OMIM] |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P08034 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. SIZE: 283 amino acids; 32025 Da SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. DISEASE: SwissProt: P08034 # Defects in GJB1 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1) [MIM:302800]; also designated CMT- X. CMTX1 is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot- Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy. CMTX1 has both demyelinating and axonal features. Central nervous system involvement may occur. & Defects in GJB1 may contribute to the phenotype of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine- Sottas syndrome. SIMILARITY: SwissProt: P08034 ## Belongs to the connexin family. Beta-type (group I) subfamily. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in convenient undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |