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Millipore Anti-Ly-6g (Mouse), Violetfluor™ 450, Clone Rb6-8c5 Antibody Is An Antibody Against Ly-6g For Use In Flow Cytometry. - Mill (Additional S&H or Hazmat Fees May Apply)
The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
Product Information
Format
violetFluor™ 450
Presentation
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality Level
MQ100
Applications
Application
Anti-Ly-6G (mouse), violetFluor™ 450, clone RB6-8C5 Antibody is an antibody against Ly-6G for use in Flow Cytometry.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
RB6-8C5
Concentration
Please refer to lot specific datasheet.
Host
Rat
Isotype
IgG2bκ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
Ly6g
Purification Method
Purified by size-exclusion chromatography
UniProt Number
P35461
Product Usage Statements
Quality Assurance
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.06 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
