| Description | |
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| Catalogue Number | S7802 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | CpG WIZ® p15 -Methylation specific PCR assay |
| Overview | Aberrant methylation of the p15 promoter region has been shown to cause inactivation in several gliomas and leukemias. The CpG WIZ® p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unmethylated) for p15* are also included. Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA. *p15 is covered under U.S. patents 5,994,095, US 6,140,473, 6,180,776, 6,210,949 and 6,218,146. |
| Product Information | |
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| Quality Level | MQ100 |
| Applications | |
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| Application | The CpG WIZ p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). |
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| Application Notes | Principles of the Technique Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the The CpG WIZ® p15 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions. |
| Biological Information | |
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| Species Reactivity |
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| Entrez Gene Number |
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| Entrez Gene Summary | This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P42772 # Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest. SIZE: 138 amino acids; 14722 Da SUBUNIT: Heterodimer of CDKN2B with CDK4 or CDK6. DISEASE:SwissProt: P42772 # Defects in CDKN2B are involved in tumor formation. SIMILARITY: SwissProt: P42772 ## Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. & Contains 4 ANK repeats. |
| Product Usage Statements | |
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| Packaging Information | |
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| Material Size | 25 assays |