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Immobilon PVDF Membranes
Western Blotting Transfer Membranes
Polyvinylidene fluoride (PVDF) membranes bind biomolecules through hydrophobic interactions. Membrane pores increase the surface binding area while restricting sizes of bound proteins.
Immobilon® PVDF (Polyvinylidene Difluoride) membranes are available in 4 types, each optimized for a specific protein blotting application. Blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper are also available.
Immobilon®-E membrane is the only PVDF membrane (0.45 µm) that wets out in aqueous buffers, eliminating the methanol pre-wet step. This membrane is well-suited for most Western blotting applications.
Immobilon®-P membrane is optimized for Western blotting and offer better handling and staining than nitrocellulose. All Immobilon®-P PVDF membranes have a pore size of 0.45 μm.
Immobilon®-PSQ membrane (0.2 µm) is ideal for protein sequencing and immunoblotting of low molecular weight proteins. It has a higher protein binding capacity and a higher retention than 0.45 µm membranes.
Immobilon®-FL membrane(0.45 µm) was developed for fluorescence-based immunodetection. It has low background fluorescence across a wide range of excitation and emission wavelengths.
Immobilon® NOW membrane has convenient pre-cut sheets with the flexibility of rolls in each format: Immobilon®-E, Immobilon®-P, Immobilon®-FL and Immobilon®-PSQ membranes.
New format Immobilon membranes
| Format | Membrane Type | Product Name | Mfg Part# | Neta Part#: | UM: | Qty: | Product Size: | |
|---|---|---|---|---|---|---|---|---|
| Immobilon-E | Immobilon-E PVDF Membrane | Millipore Immobilon-E, 8.5 cm x 10 m roll - MILL | IEVH85R | MILL-IEVH85R | EA | 1 | 8.5 cm x 10 m roll |  |
| Immobilon-FL | Immobilon-FL PVDF Membrane | Millipore Immobilon-FL, 8.5 cm x 10 m roll - MILL | IPFL85R | MILL-IPFL85R | EA | 1 | 8.5 cm x 10 m roll | |
| Immobilon-P | Immobilon-P PVDF Membrane | Millipore Immobilon-P, 8.5 cm x 10 m roll - MILL | IPVH85R | MILL-IPVH85R | EA | 1 | 8.5 cm x 10 m roll | |
| Immobilon-PSQ | Immobilon-PSQ PVDF Membrane | Millipore Immobilon-PSQ, 8.5 cm x 10 m roll - MILL | ISEQ85R | MILL-ISEQ85R | EA | 1 | 8.5 cm x 10 m roll | |
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Bundle with dispenser included
| Format | Membrane | Product Name | Mfg Part# | Neta Part#: | UM: | Qty: | Product Size: | |
|---|---|---|---|---|---|---|---|---|
| Immobilon-FL | Immobilon-FL NOW Bundle | Millipore NOW Immobilon-FL Bundle - MILL | NWKITFL | MILL-NWKITFL | EA | 1 | 8.5 cm x 10 m roll | |
| Immobilon-E | Immobilon-E NOW Bundle | Millipore NOW Immobilon-E Bundle - MILL | NWKITIE | MILL-NWKITIE | EA | 1 | 8.5 cm x 10 m roll | |
| Immobilon-P | Immobilon-P NOW Bundle | Millipore NOW Immobilon-P Bundle - MILL | NWKITIP | MILL-NWKITIP | EA | 1 | 8.5 cm x 10 m roll | |
| Immobilon-PSQ | Immobilon-PSQ NOW Bundle | Millipore NOW Immobilon-PSQ Bundle - MILL | NWKITSQ | MILL-NWKITSQ | EA | 1 | 8.5 cm x 10 m roll | |
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Immobilon NOW Dispenser
| Format | Membrane | Product Name | Mfg Part# | Neta Part#: | UM: | Qty: | Product Size: | |
|---|---|---|---|---|---|---|---|---|
| IMDISP | N/A | Millipore Immobilon NOW Dispenser - MILL | IMDISP | MILL-IMDISP | EA | 1 | N/A | |
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