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RPI X-Gal [5-Bromo-4-Chloro-3-Indolyl
RPI X-GAL [5-Bromo-4-chloro-3-indolyl-β-D-galactoside], 250 Milligrams - RPI
List Price
$47.30
Your Price
$47.30
HOW MUCH YOU SAVE:
0.00 %
NETA PART: | RPI-B71800-0.25 |
MFG.PART: | B71800-0.25 |
UNSPSC: | 12352204 |
Manufacturer: | RPI |
RPI X-GAL [5-Bromo-4-chloro-3-indolyl-β-D-galactoside], 250 Milligrams; Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. Coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture - RPI
Water Content: 0.05% (K. Fisher)
Protect from light and moisture - RPI
SKU | RPI-B71800-0.25 |
---|---|
Supplier Part Number | B71800-0.25 |
UM | UNIT |
UNSPSC | 12352204 |
Manufacturer Name | RPI |
CountryOfOrigin | IT |
ProductLine | RPI |
Qty | 1 |
MinOrderQty | 1 |
Width | 1.3 in |
Depth | 1.3 |
Height | 1.8 in |
Weight | 7.00 |
Lead Time | 3 Business Days |
Hazardous | N |
CAS Number | 7240-90-6 |