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Genscript Benz-Neburase™, His
Genscript Benz-Neburase™, His - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$39.00
Your Price
$39.00
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NETA PART: | GSCRPT-Z03626-10 |
MFG.PART: | Z03626-10 |
UNSPSC: | 12352202 |
Manufacturer: | GenScript USA Inc |
Properties
Expression System | E.coli |
Species | Serratia Marcescen |
Tag | N-terminal 6 × His Tag |
Theoretical Molecular Weight | 27.5 kDa |
Biological Activity | ≥ 1.1×106U/mg; One unitof Benz-Neburase™, His is defined as the amount of enzyme that causes a ∆A260of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30 min. |
Formulation | Suppliedas a solution of 20 mM Tris-HCl, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol, pH 8.0. |
Apparent Molecular Weight | ~27.5 kDa, on SDS-PAGEunder reducing conditions |
Storage & Stability | This product remains stable for up to 2 weeks at 4 °C or up to 24 months at -20 °C. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
Applications
Thisenzyme can be used to remove all forms of DNA and RNA, including doublestranded, single stranded, linearized, and circular forms. | |
Theactivity of Benz-Neburase™, His requires 1-2 mM Mg2+. |
Quality Control Specifications
Purity | ≥ 95% as analyzed by reducing SDS-PAGE |
Enzyme Activity | ≥250 U/μL |
Endotoxin Level | ≤ 0.1 EU/kU as determined by gel clotting method. |
Background
Target Background | The Benz-Neburase is agenetically engineered endonuclease from Serratiamarcescens used as a DNA eraser in the purification processes of biologicalmolecules. The enzyme cleaves all forms of DNA and RNA into smaller nucleotidesof around 5-8 base pairs. Benz-Neburase requires divalent cation, preferably Mg2+for activity, displays a broad pH tolerance, ranging from pH 6 to pH10, optimalat 8-8.5, and has a wide temperature tolerance, ranging from 35℃ to 44℃. The nuclease is a physiologichomodimer and functions more progressively than the monomer. Two disulfidebonds in the nuclease are crucial to its activity and stability. The enzyme isactive in a broad range of conditions and is free of proteolytic activity. Thismakes the enzyme especially useful for applications with challenging DNAcontents such as lysed host cells in viral vector manufacturing processes. |
Synonyms | alternative to Benzonase® Benzonaseis a registered trademark of Merck KGaA |
References | 1. Nestle, Marion, and W. K. Roberts. "Anextracellular nuclease from Serratia marcescens: I. Purification and someproperties of the enzyme." Journal of Biological Chemistry 244.19(1969): 5213-5218. 2. Benedik, Michael J., and Ulrich Strych."Serratia marcescens and its extracellular nuclease." FEMSmicrobiology letters 165.1 (1998): 1-13. 3. Filimonova, Maria N., Kurt L. Krause, andMichael J. Benedik. "Kinetic studies of the Serratia marcescensextracellular nuclease isoforms." Biochemistry and molecularbiology international 33.6 (1994): 122-1032. 4. Friedhoff, Peter, et al. "A procedure forrenaturation and purification of the extracellular Serratia marcescens nucleasefrom genetically engineered Escherichia coli." Protein expressionand purification 5.1 (1994): 37-43. 5. Franke, Ingo, GregorMeiss, and Alfred Pingoud. "On the Advantage of Being a Dimer, a CaseStudy Using the Dimeric Serratia Nuclease and the Monomeric Nuclease from Anabaenasp. Strain PCC 7120." Journal of Biological Chemistry 274.2(1999): 825-832. |
For laboratory research use only. Direct human use,including taking orally and injection and clinical use are forbidden.
SKU | GSCRPT-Z03626-10 |
---|---|
Supplier Part Number | Z03626-10 |
UM | EA |
UNSPSC | 12352202 |
Manufacturer Name | GenScript USA Inc |
ProductLine | GSCRPT |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 7 Business Days |