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Cell Signaling Rev-Erbalpha (E1y6d) Rabbit mAb
Cell Signaling Rev-Erbalpha (E1y6d) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
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$327.00
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$327.00
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NETA PART: | CSIG-13418S |
MFG.PART: | 13418S |
UNSPSC: | 12352202 |
Manufacturer: | Cell Signaling |
Size | 100 µl |
Reactivity | H M R |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 78 |
Source/Isotype | Rabbit IgG |
Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100, Chromatin IP: 1:100} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | Rev-Erbα (E1Y6D) Rabbit mAb recognizes endogenous levels of total Rev-erbα protein. This antibody may cross-react with an unidentified protein of 60 kDa. |
Species Reactivity | Human, Mouse, Rat |
Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser67 of human Rev-erbα protein. |
Background | Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins (1). Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells (2). In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm (3-7). Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK-3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock (8). Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2 (9). Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription (10). |
SKU | CSIG-13418S |
---|---|
Supplier Part Number | 13418S |
UM | UNIT |
UNSPSC | 12352202 |
Manufacturer Name | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |