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Cell Signaling Phospho-Lat (Tyr220) (E3s5l) Rabbit mAb
Cell Signaling Phospho-Lat (Tyr220) (E3s5l) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
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$387.00
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$387.00
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| NETA PART: | CSIG-20172S |
| MFG.PART: | 20172S |
| UNSPSC: | 12352203 |
| Manufacturer: | Cell Signaling |
| Size | 100 µl |
| Reactivity | H M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:200} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-LAT (Tyr220) (E3S5L) Rabbit mAb recognizes endogenous levels of LAT protein only when phosphorylated at Tyr220. This antibody shows cross-reactivity with phosphorylated EGFR, and detects a 70 kDa band of unknown origin in some treated cell lines. Tyr220 of LAT, long isoform corresponds to Tyr191 of LAT, short isoform. |
| Species Reactivity | Human, Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr220 of human LAT protein. |
| Background | LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4). |
| SKU | CSIG-20172S |
|---|---|
| Supplier Part Number | 20172S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |