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Cell Signaling Ras-Grf1 Antibody
Cell Signaling Ras-Grf1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$302.00
Your Price
$302.00
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NETA PART: | CSIG-3322S |
MFG.PART: | 3322S |
UNSPSC: | 12352203 |
Manufacturer: | Cell Signaling |
Size | 100 µl |
Reactivity | M |
Sensitivity | Transfected Only |
Molecular Weight (kDa) | 155 |
Source/Isotype | Rabbit |
Application/Dilution | {Western Blotting: 1:1000} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | Ras-GRF1 Antibody detects transfected levels of Ras-GRF1. This antibody does not cross-react with Ras-GRF2. |
Species Reactivity | Mouse |
Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of mouse Ras-GRF1. Antibodies are purified by protein A and peptide affinity chromatography. |
Background | Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx (1,2). Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity (1,2). Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity (3). Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity (4). It has been reported that Ras-GRF1 also shows GEF activity toward Rac after phosphorylation by the tyrosine kinase Src (5). |
SKU | CSIG-3322S |
---|---|
Supplier Part Number | 3322S |
UM | EA |
UNSPSC | 12352203 |
Manufacturer Name | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |