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Genscript THE™ His Tag Antibody, mAb, Mouse
Genscript THE™ His Tag Antibody, mAb, Mouse - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$385.00
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$385.00
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NETA PART: | GSCRPT-A00186-100 |
MFG.PART: | A00186-100 |
UNSPSC: | 12352203 |
Manufacturer: | GenScript USA Inc |
Overview
Specificity | THE™His Tag Antibody, mAb, Mouse recognizes His tags localized at the C-terminal,N-terminal, and internal region of fusion proteins. |
Host Species | Mouse |
Immunogen | Asynthetic peptide HHHHHH coupled to KLH |
Conjugate | Unconjugated |
Applications
Workingconcentrations for specific applications should be determined by theinvestigator. The appropriate concentrations may be affected by secondaryantibody affinity, antigen concentration, the sensitivity of the method ofdetection, temperature, the length of the incubations, and other factors. Thesuitability of this antibody for applications other than those listed below hasnot been determined. The following concentration ranges are recommendedstarting points for this product. | |||||||||||||||
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Properties
Form | Lyophilized |
Storage Buffer | Lyophilizedwith PBS, pH 7.4, containing 0.02% sodium azide. |
Reconstitution | Reconstitutethe lyophilized powder with sterile water (or equivalent) to a finalconcentration of 0.5 mg/mL. |
Storage Instructions | Thelyophilized product remains stable up to 1 year at -20°C from date of receipt. The reconstituted antibody can be stored for2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles. |
Purification | Purifiedby Protein A affinity chromatography. |
Isotype | Mouse IgG1,κ |
Clonality | Monoclonal |
Clone ID | 6G2A9 |
Note | GenScriptcan offer this product according to your requirement, including product size,buffer components, etc. |
Background
Target Background | Monoclonalantibodies specific to six histidine tag can greatly improve the effectivenessof several different kinds of immunoassays, helping researchers identify,detect, and purify polyhistidine fusion proteins in bacteria, insect cells, andmammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to beconjugated to KLH or some other carrier as an immunogen. After hundreds ofselection cycles, researchers at GenScript successfully isolated an antibodyagainst the His-tag. THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has veryhigh affinity towards the His-tag. Tests performed at GenScript show that theantibody can also recognize 4xHis- and 5xHis-tags.This means that even if the6xHis-tag is only partially exposed, it will still be recognized and bound bythis antibody. This antibody recognizes native as well as denatured forms ofsynthetic polyhistidine and polyhistidine-tagged fusion proteins. The productreacts with fusion proteins expressed in bacteria, insect cells, and mammaliancells. THE™ His Tag mAb recognizes His tags placed at N-terminal, C-terminal,and internal regions of fusion proteins. THE™ His Tag mAb can be used inWestern blot analyses, Dot blot analyses, ELISA, immunofluorescent staining,and flow cytometry of cultured cells. |
Synonyms | THE™Anti-His mAb |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
SKU | GSCRPT-A00186-100 |
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Supplier Part Number | A00186-100 |
UM | EA |
UNSPSC | 12352203 |
Manufacturer Name | GenScript USA Inc |
ProductLine | GSCRPT |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 7 Business Days |