Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1691-25UG |
| Description | Anti-BLUE Antibody, clone 2D2-F11 |
| Background Information | Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-Blue antibody, Clone 2D2-F11, is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. The use of this antibody allows for visualization of marker proteins along with the protein of interest on the same blot. Anti-Blue antibody offers high specificity and does not interfere with the detection of other antibodies, In addition, it does not display reactivity with other cellular proteins, unstained marker proteins, or with proteins stained with different dyes. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-BLUE, clone 2D2-F11 Antibody, Cat. No. MABC1691-25UG, validated for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected BLUE in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363). |
| Biological Information | |
|---|---|
| Immunogen | Prestained Precision Plus Protein marker proteins. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Anti-BLUE, clone 2D2-F11, is a mouse monoclonal antibody that detects prestained Precision Plus Protein marker proteins. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | All. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein G purified |
| UniProt Number |
|
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Pre-Stained Protein Standard. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |