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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC544 |
| Description | Anti-PLK-4 Antibody, clone 6H5 |
| Alternate Names |
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| Background Information | Serine/threonine-protein kinase PLK4 (PLK-4) is a member of the protein kinase superfamily and plays an essential role in centriole duplication. PLK-4 triggers procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins. It may be involved in tumorigenesis since centrosome aberrations are frequently observed in tumors. PLK-4 may also be involved in trophoblast differentiation by phosphorylating HAND1, leading to the disruption between HAND1 and MDFIC and then activating HAND1. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Polo Like Kinase (PLK) using this mouse monoclonal antibody, Anti-PLK-4 Antibody, clone 6H5 validated for use in western blotting, ICC & IP. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected PLK-4 in U20S cell lysates transfected with control and Cep152 siRNA, but not U2OS cell lysates transfected with PLK4 siRNA. (Cizmecioglu, O. et al. (2010). J Cell Biol. 191(4):731-739). Immunoprecipitation Analysis: A representative lot immunoprecipitated PLK-4 in HeLa cell lysates cotransfected with Flag-tagged Gcp6 and Myc-tagged Plk-4 (Bahtz, R. et al. (2012). J Cell Sci. 125 (Pt 2): 486-496). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to Human PLK-4. |
| Clone | 6H5 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG3κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | 109 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in U2Os cell lysate. Western Blotting Analysis: 4.0 µg/mL of this antibody detected PLK-4 in 10 µg of U2Os cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |