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Millipore This Mouse Monoclonal Antibody Anti-Cd86 (B7-2) (Human), Pe, Clone It2.2 Validated For Use In Western Blotting, Ihc & Flow Cytometry For The Detection Of Cd86 (B7-2). - Mill (Additional S&H or Hazmat Fees May Apply)
The IT2.2 antibody reacts with human CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.The IT2.2 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells. It is reported to be cross-reactive with Rhesus, Cynomolgus and Common marmoset CD86.
Product Information
Format
PE
Presentation
Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
This mouse monoclonal antibody Anti-CD86 (B7-2) (human), PE, clone IT2.2 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD86 (B7-2).
Key Applications
Immunohistochemistry
Immunoprecipitation
Flow Cytometry
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
IT2.2
Concentration
5 µL (0.5 µg)/test
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
CD86
CD28LG2
Purification Method
Size Exclusion Chromatography
UniProt Number
P42081
Product Usage Statements
Quality Assurance
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.5 µg of this antibody detected CD86 (B7-2) in one million human peripheral blood monocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
