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Millipore This Mouse Monoclonal Antibody Anti-Cd25 (Human), Pe-Cy7 & Clone Bc96 Validated For Use In Flow Cytometry For The Detection Of Cd25. - Mill (Additional S&H or Hazmat Fees May Apply)
The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells.The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on “induced” peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.
Product Information
Format
PE-Cy7
Presentation
Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
This mouse monoclonal antibody Anti-CD25 (human), PE-Cy7 & clone BC96 validated for use in Flow Cytometry for the detection of CD25.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
BC96
Concentration
5 µL (0.25 µg)/test
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
IL2RA
Purification Method
Size Exclusion Chromatography
UniProt Number
P01589
Product Usage Statements
Quality Assurance
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.25 µg of this antibody detected CD25 in one million human peripheral blood lymphocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.