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Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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M | WB | M | Purified | Monoclonal Antibody |
Description | |
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Catalogue Number | MABN748 |
Description | Anti-TrpC3, clone 10H6 Antibody |
Alternate Names |
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Background Information | TrpC3, also known as Short transient receptor potential channel 3, transient receptor protein 3 or TRP-3, and encoded by the gene TRPC3/TRP3, forms a receptor activated calcium channel that is activated via ligand binding and transduces signals via the G-protein coupled tyrosine receptor kinases pathways.TrpC3 can also be activated by the release of internal calcium and is one of the principal receptors involved in maintaining Ca++ ion homeostasis in skeletal muscles and nervous tissue. TrpC3 is also a principal receptor involved in the Capacitative calcium entry (CCE) which is the mechanism behind Ca++ influx into cells that replenishes internal calcium stores. Additionally, TrpC3 receptor is important in axon guidance, phototransduction, and platelet activation. TrpC3 is localized to the cell membrane and consists of a multipass-though transport protein. TrpC3 is expressed principally in the brain but is also found in most other tissues and organs at lowered levels. |
Product Information | |
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Format | Purified |
Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
Quality Level | MQ100 |
Applications | |
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Application | This Anti-TrpC3, clone 10H6 Antibody is validated for use in Western Blotting for the detection of TrpC3. |
Key Applications |
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Application Notes | Western Blotting Analysis: A representative lot detected TrpC3 in cerebellar tissues of TRPC3 wild type mice and not TRPC3 knock out mice (Prof. Yizheng Wang, Shanghai Institutes for Biological Sciences (SIBS). Western Blotting Analysis: A representative lot detected TrpC3 in rat liver and brain tissue lysates (Feng. S., et al. (2013). PNAS. 1-6). |
Biological Information | |
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Immunogen | Recombinant protein corresponding to mouse TrpC3. |
Clone | 10H6 |
Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
Host | Mouse |
Isotype | IgG1 |
Species Reactivity |
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Species Reactivity Note | Mouse. Predicted to react with Human based on 100% sequence homology. |
Antibody Type | Monoclonal Antibody |
Entrez Gene Number |
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Gene Symbol |
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Purification Method | Protein G Purified |
UniProt Number |
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Molecular Weight | ~110 kDa observed. |
Product Usage Statements | |
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Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected TrpC3 in 10 µg of mouse brain tissue lysate. |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
Packaging Information | |
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Material Size | 100 µg |