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Cell Signaling Phospho-Hsp27 (Ser82) (D1h2f6) Xp Rabbit mAb
Cell Signaling Phospho-Hsp27 (Ser82) (D1h2f6) Xp Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
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$170.00
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$170.00
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| NETA PART: | CSIG-9709T |
| MFG.PART: | 9709T |
| UNSPSC: | 41116012 |
| Manufacturer: | Cell Signaling |
| Size | 20 µl |
| Reactivity | H M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 27 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:50 - 1:200, Immunofluorescence (Immunocytochemistry): 1:50 - 1:200, Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #64293. |
| Specificity/Sensitivity | Phospho-HSP27 (Ser82) (D1H2F6) XP® Rabbit mAb recognizes endogenous levels of HSP27 protein only when phosphorylated at Ser82. |
| Species Reactivity | Human, Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27 protein. |
| Background | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). |
| SKU | CSIG-9709T |
|---|---|
| Supplier Part Number | 9709T |
| UM | UNIT |
| UNSPSC | 41116012 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |