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Genscript GenCRISPR™Ultra eSpCas9-2NLS-basic GMP
Genscript GenCRISPR™Ultra eSpCas9-2NLS-basic GMP - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$209.00
Your Price
$209.00
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NETA PART: | GSCRPT-Z03624-100 |
MFG.PART: | Z03624-100 |
UNSPSC: | 12352204 |
Manufacturer: | GenScript USA Inc |
Overview
Description | GenCRISPR™ Ultra eSpCas9-2NLS-basic GMP isutilized for CRISPR gene editing applications. The Cas9 nuclease forms a stableribonucleoprotein (RNP) complex with the guide RNA (gRNA) component. With thehelp of two nuclear localization signals (NLS) expressed with the Cas9nuclease, the RNP complex enters the nucleus and cleaves target gene. Whencompared with a plasmid-based delivery system, the RNP delivery system has beenobserved to increase the on-target gene editing efficiency and decreaseoff-target effects. | ||||||
Selection Guide | GenScript provides two types of Cas9nucleases in basic GMP* for selection based on your specific downstreamapplications.
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Applications
sgRNA-dependentdouble-stranded DNA cleavage. |
Properties
Source | Recombinant mutant Cas9 with nuclearlocalization signal (NLS) at both N-terminal and C-terminal expressed in E.coli |
Species | S. pyogenes |
Tag | Tag-free |
Theoretical Molecular Weight | ~160 kDa |
Concentration | 10 mg/ml |
Active temperature | optimal at 37 °C. |
Storage Buffer | 25 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 50%Glycerol, pH 8.0. |
Storage & Stability | Store at-20 °C for up to 12months from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
Quality Control Specifications
Appearance | Clear, colorless, liquid |
Purity | ≥ 95% as analyzed by SDS-PAGE ≥ 95% as analyzed by SEC-HPLC |
Concentration by A280 | 10 mg/ml ± 1 mg/ml |
Bioactivity (in vitro) | ≥ 85% |
Residual DNase | ≤ 10 ng/mg |
Residual RNase | ≤ 1 ng/mg |
Endotoxin Level | ≤ 10 EU/mg |
Residual HCP | ≤ 10 ng/mg |
Residual HCD | ≤ 1 ng/mg |
Bioburden | < 1 CFU/ml |
Mycoplasma | < LOD |
Background
References | 1. Jinek, Martin, et al. "A programmable dual-RNA–guidedDNA endonuclease in adaptive bacterial immunity." science 337.6096(2012): 816-821. |
Annotation | *basicGMP isa branding term that GenScript uses to describe reagents manufactured incompliance with ISO 9001 and ISO 13485 quality management system standards andwith more stringent process controls and relatively complete documentationrecords. GenScript is capable of providing documents, site audit and othersupport to help with the applications of your projectsin specific regions. |
For research ormanufacturing use. Not intended for clinical use. Direct human use, includingtaking orally and injection are forbidden.
SKU | GSCRPT-Z03624-100 |
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Supplier Part Number | Z03624-100 |
UM | UN |
UNSPSC | 12352204 |
Manufacturer Name | GenScript USA Inc |
ProductLine | GSCRPT |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 7 Business Days |