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Genscript GenCRISPR™ LbuCas13a Nuclease
Genscript GenCRISPR™ LbuCas13a Nuclease - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$189.00
Your Price
$189.00
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NETA PART: | GSCRPT-Z03742-100 |
MFG.PART: | Z03742-100 |
UNSPSC: | 12352204 |
Manufacturer: | GenScript USA Inc |
Overview
Description | GenCRISPR™ LbuCas13a Nuclease is an RNA-guidedRNA endonuclease from Leptotrichiabuccalis. Cas13a (previously known as C2c2) belongs to the Class 2 Type VI CRISPR-Cas system andcontains a single protein effector with two HEPN domains. Cas13a exhibits twodistinct ribonuclease activities. Cas13a possesses sequence-specific RNAcleavage activity in vitro and in vivo. And after specific cleavage, Cas13aactivates collateral cleavage activity towards adjacent non-specific ssRNAsequences. The two distinct ribonuclease activities of Cas13a enable guide-RNAprocessing and RNA detection. The RNA detection empowered with Cas13a-crRNA complexand quenched fluorescent ssRNA as reporter has been reported to be versatile,rapid and high-specific. |
Applications
crRNA-dependent RNA cleavage |
Properties
Source | Recombinant Cas13a expressed by E.coli |
Species | Leptotrichia buccalis |
Tag | Tag-free |
Apparent Molecular Weight | ~140 kDa, on SDS-PAGE under non-reducingconditions |
Concentration | Please refer to the COA for the specific lot. |
Storage Buffer | 10 mM Tris-HCl, 300 mM NaCl, 1.0 mM DTT, 50%glycerol, pH 8.0. |
Storage & Stability | Storeat -20 °C for up to 12 months from the date of manufacture. Avoid repeatedfreeze-thaw cycles. Do not store below-20 °C! |
Accession# | C7NBY4 |
Quality Control Specifications
Appearance | Clear, colorless liquid |
Purity | ≥ 90% by SDS-PAGE |
Activity | ≥ 85% by invitro digestion of ssRNA |
Concentration | 4 mg/ml ± 10% as analyzed by A280 |
Residual DNase | Undetectable |
Residual RNase | Undetectable |
Endotoxin Level | < 0.2 EU/μg |
Background
References | 1. Abudayyeh, Omar O., et al. "C2c2 is a single-componentprogrammable RNA-guided RNA-targeting CRISPR effector." Science 353.6299(2016). |
For laboratory research use only. Direct human use,including taking orally and injection and clinical use are forbidden.
SKU | GSCRPT-Z03742-100 |
---|---|
Supplier Part Number | Z03742-100 |
UM | UN |
UNSPSC | 12352204 |
Manufacturer Name | GenScript USA Inc |
ProductLine | GSCRPT |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 7 Business Days |