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Genscript GenCRISPR LbCas12a Nuclease
Genscript GenCRISPR™ LbCas12a Nuclease,100ug - GSCRPT (Additional S&H or Hazmat Fees May Apply)
List Price
$169.00
Your Price
$169.00
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NETA PART: | GSCRPT-Z03753-100 |
MFG.PART: | Z03753-100 |
UNSPSC: | 12352204 |
Manufacturer: | GenScript USA Inc |
Overview
Description | GenCRISPR™ LbCas12a Nuclease is anRNA-guided DNA endonuclease from Lachnospiraceaebacterium. Cas12a (previously known as Cpf1) belongs to the Class 2 Type V CRISPR/Cassystem. Different from CRISPR/Cas9 system, the ribonucleoprotein (RNP) complexof CRISPR/Cas12a system is formed by Cas12a and crRNA, it recognizes a T-rich protospaceradjacent motif (PAM) and results in a staggered DNA double-strand break (DSB). Afterthe specific cleavage, Cas12a can also activatecollateral cleavage activity towards adjacent non-specific ssDNA sequences. Hence, Cas12a nuclease is a good alternative for Cas9 in certaintarget DNA editing, and provides a novel strategy for DNA detection. |
Applications
crRNA-dependent DNA cleavage |
Properties
Source | Recombinant Cas12a with a C-terminal NLSexpressed by E.coli |
Species | Lachnospiraceae bacterium |
Tag | C-terminal 6× His Tag |
Apparent Molecular Weight | 146 kDa, on SDS-PAGE under non-reducingconditions |
Concentration | 4 mg/ml |
Storage Buffer | 10 mM Tris-HCl, 300 mM NaCl, 0.5 mM DTT, 50%glycerol, pH 7.4. |
Storage & Stability | Storeat -20 °C for up to 12 months from the date of manufacture. Avoid repeatedfreeze-thaw cycles. Do not store below-20 °C! |
Accession# | 6KL9_A |
Quality Control Specifications
Appearance | Clear, colorless liquid |
Purity | ≥ 90% by SDS-PAGE |
Concentration by A280 | 4(± 10%) mg/ml |
Bioactivity (in vitro) | ≥ 90% |
Residual DNase | Undetectable |
Residual RNase | Undetectable |
Endotoxin Level | < 0.1 EU/μg |
Background
References | 1. Zetsche, Bernd, et al."Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cassystem." Cell 163.3 (2015): 759-771. 2. Ledford, Heidi. "Alternative CRISPR system could improvegenome editing." Nature News526.7571 (2015): 17. 3. Chen, Janice S., et al."CRISPR-Cas12a target binding unleashes indiscriminate single-strandedDNase activity." Science360.6387 (2018): 436-439. |
For laboratory research use only. Direct human use,including taking orally and injection and clinical use are forbidden.
SKU | GSCRPT-Z03753-100 |
---|---|
Supplier Part Number | Z03753-100 |
UM | UN |
UNSPSC | 12352204 |
Manufacturer Name | GenScript USA Inc |
CountryOfOrigin | CN |
ProductLine | GSCRPT |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 7 Business Days |