| Description | |
|---|---|
| Catalogue Number | 14-485 |
| Brand Family | Upstate |
| Trade Name |
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| Description | IKKβ Protein, active, 10 µg |
| Overview | Recombinant, full-length human IKKβ, containing N-terminal His6 tag |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, recombinant, full-length human IKKβ, containing N-terminal His6 tag, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Expressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] |
| Gene Symbol |
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| Protein Target | IKKβ |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | Misc |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O14920 # Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3 (By similarity). SIZE: 756 amino acids; 86564 Da SUBUNIT: Preferentially found as a heterodimer with IKK-alpha but also as a homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. The tripartite complex can also bind to MEKK1, MAP3K14/NIK, IKAP and IKB-alpha-p65-p50 complex. Phosphorylated IKB-alpha is further released from the complex. Found in a complex composed of NCOA2, NCOA3, IKKA, IKBKG and CREBBP. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with Yersinia yopJ. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood. PTM: Upon cytokine stimulation, phosphorylated on Ser-177 and Ser- 181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. & Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway. SIMILARITY: SwissProt: O14920 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 88kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of IKKtide |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 1 year at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250μg size -- call for pricing and availability and reference catalog number 14-485M when ordering the 250μg size. |